通过反相柱色谱法对DNA限制片段进行制备性分级分离。
Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.
作者信息
Hardies S C, Wells R D
出版信息
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3117-21. doi: 10.1073/pnas.73.9.3117.
Abstract
Reversed phase column chromatography on RPC-5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undertermined property.
摘要
采用RPC - 5树脂进行反相柱色谱法，对由限制性内切酶产生的毫克量DNA片段进行分级分离。分级分离的依据是片段大小、是否存在粘性末端以及至少一种尚未确定的特性。
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