Maza L M, Carter B J
Nucleic Acids Res. 1976 Oct;3(10):2605-16. doi: 10.1093/nar/3.10.2605.
Duplex AAV-2 DNA was digested with SalI, PstI or HaeII restriction endonucleases and the cleavage sites were mapped. SalI cleaves AAV DNA at 0.310 map units, PstI at 0.106, 0.422 and 0.914 and the five HaeII sites were mapped at 0.110. 0.156, 0.181, 0.536 and 0.600 map units. These cleavage products will be useful for the isolation of specific regions from the AAV DNA, located outside of the stably transcribed region of the genome, and will also help to map more complex restriction enzyme cleavages.
双链腺相关病毒2型（AAV - 2）DNA用SalI、PstI或HaeII限制性内切酶消化，并对切割位点进行定位。SalI在0.310个图谱单位处切割AAV DNA，PstI在0.106、0.422和0.914处切割，五个HaeII位点分别定位在0.110、0.156、0.181、0.536和0.600个图谱单位处。这些切割产物将有助于从基因组稳定转录区域之外的AAV DNA中分离特定区域，也将有助于绘制更复杂的限制性内切酶切割图谱。
