Spear I S, Fife K H, Hauswirth W W, Jones C J, Berns K I
J Virol. 1977 Nov;24(2):627-34. doi: 10.1128/JVI.24.2.627-634.1977.
Duplex adeno-associated virus (AAV) DNA, produced by annealing plus and minus virion single strands, has been digested with several bacterial restriction endonucleases. These studies reveal the existence of alternate secondary structures at the termini of duplex AAV DNA. Analysis of the sites of endo R-Hpa II cleavage, the products of complete endo R-Hpa II digestion, and the multiple terminal secondary structures leads to the conclusion that there are two possible nucleotide sequences at each end of AAV DNA. A model that attributes the terminal nucleotide sequence heterogeneity to two possible orientations of the first 120 nucleotides at each end of the DNA is proposed; in one case the sequence is 1 to 120; in the other case the sequence is inverted. An origin of the inversion is suggested based on previously described intermediates in AAV DNA replication.
双链腺相关病毒（AAV）DNA是由正链和负链病毒粒子单链退火产生的，已用几种细菌限制性内切酶进行了消化。这些研究揭示了双链AAV DNA末端存在交替的二级结构。对内切酶R-Hpa II切割位点、内切酶R-Hpa II完全消化产物以及多个末端二级结构的分析得出结论，AAV DNA两端各有两种可能的核苷酸序列。提出了一个模型，将末端核苷酸序列的异质性归因于DNA两端前120个核苷酸的两种可能方向；在一种情况下，序列是1到120；在另一种情况下，序列是反向的。基于先前描述的AAV DNA复制中间体，提出了倒置的起源。
