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用细菌限制性核酸内切酶对腺相关病毒DNA精细结构的研究。

Study of the fine structure of adeno-associated virus DNA with bacterial restriction endonucleases.

作者信息

Berns K I, Kort J, Fife K H, Grogan E W, Spear I

出版信息

J Virol. 1975 Sep;16(3):712-9. doi: 10.1128/JVI.16.3.712-719.1975.

DOI:10.1128/JVI.16.3.712-719.1975
PMID:1159899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354719/
Abstract

A physical map of the adeno-associated virus type 2 genome has been constructed on the basis of the five fragments produced by the restriction endonucleases HindII + III from Hemophilus influenzae. There are three endo R-HindII cleavage sites and one endo R-HindIII site. Evidence has been obtained to support the existence of two nucleotide sequence permutations in adeno-associated virus DNA, the start points of which have been estimated to be separated by 1% of the genome. The three cleavage fragments produced by endo R-Eco RI have been ordered and oriented with respect to the endo R-HindII + III cleavage map.

摘要

基于流感嗜血杆菌的限制性内切酶HindII + III产生的五个片段构建了2型腺相关病毒基因组的物理图谱。存在三个内切酶R-HindII切割位点和一个内切酶R-HindIII位点。已获得证据支持腺相关病毒DNA中存在两种核苷酸序列排列，其起始点估计相隔基因组的1%。内切酶R-Eco RI产生的三个切割片段已相对于内切酶R-HindII + III切割图谱进行了排序和定向。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/769ad2dfdad6/jvirol00237-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/cce3f212e26c/jvirol00237-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/59859e62229b/jvirol00237-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/25e42bbe85dc/jvirol00237-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/c61c04888973/jvirol00237-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/30ed8cda7900/jvirol00237-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/769ad2dfdad6/jvirol00237-0266-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/cce3f212e26c/jvirol00237-0261-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/59859e62229b/jvirol00237-0262-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/25e42bbe85dc/jvirol00237-0263-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/c61c04888973/jvirol00237-0263-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/30ed8cda7900/jvirol00237-0265-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fc94/354719/769ad2dfdad6/jvirol00237-0266-a.jpg

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引用本文的文献

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2
Nucleotide sequence and organization of the adeno-associated virus 2 genome.腺相关病毒2型基因组的核苷酸序列与结构
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ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES.腺病毒相关缺陷病毒颗粒
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Nucleotide sequence of the inverted terminal repetition in adeno-associated virus DNA.腺相关病毒DNA中反向末端重复序列的核苷酸序列。
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Comparison of neutralization and DNA restriction enzyme methods for typing clinical isolates of human adenovirus.人腺病毒临床分离株分型的中和法与DNA限制酶法比较
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Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands.单链腺病毒相关病毒基因组的证据：互补单链的分离与纯化
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A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
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Evidence for a single-stranded adenovirus-associated virus genome: formation of a DNA density hybrid on release of viral DNA.单链腺病毒相关病毒基因组的证据：病毒DNA释放时形成DNA密度杂种。
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Separation of two types of adeno-associated virus particles containing complementary polynucleotide chains.含有互补多核苷酸链的两种腺相关病毒颗粒的分离。
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