用限制性内切酶KpnI和PstI切割多瘤病毒DNA。
The cleavage of polyoma virus DNA by restriction enzymes KpnI and PstI.
作者信息
Crawford L V, Robbins A K
出版信息
J Gen Virol. 1976 Jun;31(3):315-21. doi: 10.1099/0022-1317-31-3-315.
The action of two restriction endonucleases on polyoma virus DNA has been examined and the sites at which they cleave the DNA located. One of the enzymes, KpnI from Klebsiella pneumoniae OK8, cleaves polyoma DNA twice at about 11-6 and 59-2% from the EcoRI site. The other enzyme, PstI from Providencia stuartii 164, cleaves polyoma DNA five times at about 14-8, 16-5, 32-6, 50-3 and 80-0% from the EcoRI site. Some of the cleavages produced by these enzymes alone, or in conjunction with other endonucleases, may be of use in the isolation of regions of particular interest from the virus DNA.
研究了两种限制性内切酶对多瘤病毒DNA的作用，并确定了它们切割DNA的位点。其中一种酶是来自肺炎克雷伯菌OK8的KpnI，它在距离EcoRI位点约11-6%和59-2%处对多瘤DNA进行两次切割。另一种酶是来自斯氏普罗威登斯菌164的PstI，它在距离EcoRI位点约14-8%、16-5%、32-6%、50-3%和80-0%处对多瘤DNA进行五次切割。这些酶单独或与其他内切酶联合产生的一些切割，可能有助于从病毒DNA中分离出特别感兴趣的区域。
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