Tanaka K, Goto K
Poult Sci. 1976 Sep;55(5):1774-8. doi: 10.3382/ps.0551774.
The ovarian ruptured follicles larger than 0.2 g. of wet weight were collected from laying hens and an ovarian oviposition-inducing factor (OOIF) was extracted from the follicles. The extract was chromatographed on a column of Sephadex G-75. Material from the active region for inducing premature oviposition was subjected to thioglycollate or trypsin treatment. It was found that oxytocic activity of this material was completely destroyed by thioglycollate buy not by trypsin, when tested on the contractility of the isolated hen's uterus. The OOIF was found to be diffusible through a cellophane bag. It is suggested that the OOIF may be a relatively small molecule have a disulfied bridge and it seems to be a hormone or hormone-like substance resembling the posterior pituitary hormones in the chemical nature.
从产蛋母鸡中收集湿重超过0.2克的卵巢破裂卵泡，并从卵泡中提取卵巢排卵诱导因子（OOIF）。提取物在Sephadex G - 75柱上进行色谱分析。将诱导过早排卵的活性区域的物质用巯基乙酸盐或胰蛋白酶处理。当在分离的母鸡子宫收缩性上进行测试时，发现该物质的催产活性被巯基乙酸盐完全破坏，但不被胰蛋白酶破坏。发现OOIF可透过玻璃纸袋扩散。有人提出，OOIF可能是一个相对较小的分子，具有二硫键，并且在化学性质上似乎是一种类似于垂体后叶激素的激素或激素样物质。
