Heiland B, Greenfield S A
University Department of Pharmacology, Oxford, UK.
Pharmacol Biochem Behav. 1999 Jan;62(1):81-7. doi: 10.1016/s0091-3057(98)00117-8.
In the substantia nigra acetylcholinesterase is released from the dopamine cells of the pars compacta independent of cholinergic transmission. In this study the effects of local and systemic amphetamine treatment were compared on acetylcholinesterase release in the rat substantia nigra in relation to concomitant behavior. Acetylcholinesterase release, measured "on-line" with a sensitive chemiluminescent system, was enhanced by amphetamine stimulation administered locally and could not be dissociated from simultaneous amphetamine-induced circling behavior. On the other hand, amphetamine administered systemically resulted in a general increase in locomotor behavior followed by a subsequent increase in acetylcholinesterase release. The alternative scenario of an initial rise in acetylcholinesterase release, subsequently followed by enhanced movement, was never seen. Hence, movement can enhance release of acetylcholinesterase from the substantia nigra, whereas "upstream" local nigral events can affect acetylcholinesterase release and movement simultaneously.
在黑质中，乙酰胆碱酯酶从致密部的多巴胺能细胞释放，与胆碱能传递无关。在本研究中，比较了局部和全身给予苯丙胺对大鼠黑质中乙酰胆碱酯酶释放的影响及其伴随行为。用灵敏的化学发光系统“在线”测量，局部给予苯丙胺刺激可增强乙酰胆碱酯酶的释放，且与同时出现的苯丙胺诱导的转圈行为无法分离。另一方面，全身给予苯丙胺会导致运动行为普遍增加，随后乙酰胆碱酯酶释放增加。从未观察到乙酰胆碱酯酶释放先升高，随后运动增强的另一种情况。因此，运动可增强黑质中乙酰胆碱酯酶的释放，而“上游”的局部黑质事件可同时影响乙酰胆碱酯酶的释放和运动。
