Toda M, Miura M, Asou H, Sugiyama I, Kawase T, Uyemura K
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
Neurochem Res. 1999 Feb;24(2):339-43. doi: 10.1023/a:1022538810581.
We examined the effect of expression of glial fibrillary acidic protein (GFAP) on the tumor growth of astrocytoma in vivo. When rat astrocytoma C6 cells were injected subcutaneously in athymic mice, the cells produced tumors that grew rapidly. The tumor growth of C6 cells transfected with GFAP cDNA was significantly reduced compared to that of control NeoC6 cells transfected only with the neomycin resistant gene. After implantation of C6 cells transfected with mutated GFAP cDNA at the phosphorylation sites, the tumor growth was suppressed similar to that of the wild GFAP transfectants. To determine whether the cell growth suppression by GFAP is specific for astroglial cells, we assessed the effect of GFAP on the cell growth of an L cell of fibroblast origin in vitro. By GFAP cDNA transfection, L cells showed morphological changes, but the cell growth was not reduced. These results suggest that GFAP is a critical regulator of the tumor growth of astrocytoma.
我们研究了胶质纤维酸性蛋白（GFAP）的表达对体内星形细胞瘤肿瘤生长的影响。当将大鼠星形细胞瘤C6细胞皮下注射到无胸腺小鼠体内时，这些细胞会产生迅速生长的肿瘤。与仅转染了新霉素抗性基因的对照NeoC6细胞相比，转染了GFAP cDNA的C6细胞的肿瘤生长明显降低。在植入磷酸化位点突变的GFAP cDNA转染的C6细胞后，肿瘤生长受到抑制，类似于野生型GFAP转染细胞。为了确定GFAP对细胞生长的抑制是否对星形胶质细胞具有特异性，我们在体外评估了GFAP对成纤维细胞来源的L细胞生长的影响。通过GFAP cDNA转染，L细胞出现形态变化，但细胞生长未降低。这些结果表明，GFAP是星形细胞瘤肿瘤生长的关键调节因子。
