Department of Biotechnology, Guru Nanak Dev University, Amritsar 143005, India.
Evid Based Complement Alternat Med. 2011;2011:267614. doi: 10.1093/ecam/nep188. Epub 2011 Feb 14.
Root extracts of Withania somnifera (Ashwagandha) are commonly used as a remedy for a variety of ailments and a general tonic for overall health and longevity in the Indian traditional medicine system, Ayurveda. We undertook a study to investigate the anti-proliferative and differentiation-inducing activities in the water extract of Ashwagandha leaves (ASH-WEX) by examining in glioma cells. Preliminary detection for phytochemicals was performed by thin-layer chromatography. Cytotoxicity was determined using trypan blue and MTT assays. Expression level of an hsp70 family protein (mortalin), glial cell differentiation marker [glial fibrillary acidic protein (GFAP)] and neural cell adhesion molecule (NCAM) were analyzed by immunocytochemistry and immunoblotting. Anti-migratory assay was also done using wound-scratch assay. Expression levels of mortalin, GFAP and NCAM showed changes, subsequent to the treatment with ASH-WEX. The data support the existence of anti-proliferative, differentiation-inducing and anti-migratory/anti-metastasis activities in ASH-WEX that could be used as potentially safe and complimentary therapy for glioma.
睡茄（印度人参）的根提取物在印度传统医学体系阿育吠陀中被广泛用作治疗各种疾病的药物，以及整体健康和长寿的滋补品。我们进行了一项研究，以调查水提睡茄叶（ASH-WEX）在神经胶质瘤细胞中的抗增殖和诱导分化活性。通过薄层层析法对植物化学物质进行初步检测。使用台盼蓝和 MTT 测定法测定细胞毒性。通过免疫细胞化学和免疫印迹分析 hsp70 家族蛋白（ mortalin ）、神经胶质细胞分化标志物[胶质纤维酸性蛋白（GFAP）]和神经细胞黏附分子（NCAM）的表达水平。还使用划痕实验进行了抗迁移测定。在 ASH-WEX 处理后，mortalin、GFAP 和 NCAM 的表达水平发生了变化。这些数据支持 ASH-WEX 具有抗增殖、诱导分化以及抗迁移/抗转移活性，可作为治疗神经胶质瘤的潜在安全和辅助疗法。
