Winter M, Winter C, Wiechens B
Department of Ophthalmology, University of Kiel, Germany.
Graefes Arch Clin Exp Ophthalmol. 1999 Feb;237(2):153-6. doi: 10.1007/s004170050210.
A study was performed to determine the amount of intraocular retained perfluorodecalin after macroscopic complete removal.
Freshly enucleated pig eyes had the anterior segment removed, vitrectomy was carried out, and the eye cups were placed in 0.9% buffered saline solution. One millilitre of perfluorodecalin was instilled for 30 min, followed by a fluid-air exchange. Perfluorodecalin was macroscopically removed. The retina was rinsed twice with 0.9% buffered saline solution. In a second group no rinsing was done, while in a third group no fluid-air exchange was performed. Finally all eye cups were filled with 2.0 ml of the perfluoropolyether Hostinert 130 to dissolve retained perfluorodecalin. The quantity of perfluorodecalin in perfluoropolyether was determined by gas chromatography.
Retained perfluorodecalin was detected in all experiments. The smallest portion of perfluorodecalin retained (range 0.04-0.08, mean 0.058%, SD +/- 0.015%) was observed without fluid-air exchange. After fluid-air exchange the portion with rinsing was 0.11-0.27% (mean 0.21%, SD +/- 0.059%) and that without rinsing was 0.51-0.69% (mean 0.60%, SD +/- 0.065%).
Even after macroscopic complete removal of perfluorodecalin a thin layer remains on the retina. If intraoperative fluid-gas exchange is necessary, multiple rinsing with 0.9% buffered saline solution should be performed to reduce the amount of perfluorocarbon liquid retained.
进行了一项研究以确定宏观完全清除后眼内残留全氟萘烷的量。
将新鲜摘除的猪眼切除前段，进行玻璃体切除术，然后将眼杯置于0.9%缓冲盐溶液中。滴入1毫升全氟萘烷30分钟，随后进行液气交换。肉眼下清除全氟萘烷。视网膜用0.9%缓冲盐溶液冲洗两次。第二组不进行冲洗，第三组不进行液气交换。最后，所有眼杯均填充2.0毫升全氟聚醚Hostinert 130以溶解残留的全氟萘烷。通过气相色谱法测定全氟聚醚中全氟萘烷的含量。
在所有实验中均检测到残留的全氟萘烷。在不进行液气交换的情况下观察到残留的全氟萘烷比例最小（范围为0.04 - 0.08，平均0.058%，标准差±0.015%）。进行液气交换后，冲洗组的比例为0.11 - 0.27%（平均0.21%，标准差±0.059%），未冲洗组的比例为0.51 - 0.69%（平均0.60%，标准差±0.065%）。
即使在肉眼下完全清除全氟萘烷后，视网膜上仍会残留一层薄薄的物质。如果术中需要进行液气交换，应多次用0.9%缓冲盐溶液冲洗以减少残留的全氟碳液体量。
