Friberg Thomas R, Siska Peter E, Somayajula Kasi, Williams John, Eller Andrew W
Room 824, 203 Lothrop Street, Pittsburgh, PA 15213, USA.
Graefes Arch Clin Exp Ophthalmol. 2003 Oct;241(10):809-15. doi: 10.1007/s00417-003-0698-5. Epub 2003 Sep 11.
Perfluorocarbon liquids (PFCL) are used extensively in complex vitreoretinal surgery, sometimes before the placement of silicone oil (SiO). We suspected that PFCL and SiO interact physically when in opposition, potentially making their removal more difficult. The nature of some of these interactions was explored using a mass spectrometric approach in in-vitro and in-vivo samples.
We incubated silicone oil (1,000 or 5,000 centistokes viscosity) and PFCL [perfluoro-n-octane (PFO) or perfluorotributylamine] together in vitro for 6 months and performed electron impact ionization mass spectrometry (EIMS) on the PFCL to characterize interactions between the liquid phases. Packaged samples of PFCL served as controls. We also examined in vivo samples of PFO which had been retained in human eyes for several months prior to surgical removal.
Perfluorocarbon liquids packaged for surgical use all contain SiO in trace amounts, possibly as a manifestation of the processes used in their manufacture. Furthermore, all PFCLs incubated with SiO showed much more prominent contamination with SiO molecular fragments. PFCL was found in the SiO phase of eyes in which both liquids were present for extended periods of time. The EIMS analysis of in vivo samples suggested that proteins coat PFCL droplets, forming micelle-like structures.
Medical-grade PFCLs contain small amounts of SiO, and PFCLs dissolve small amounts of oil into solution over time. Interactions between retained vitreous substitutes may have clinical relevance.
全氟化碳液体（PFCL）广泛应用于复杂的玻璃体视网膜手术中，有时在注入硅油（SiO）之前使用。我们怀疑PFCL和SiO在相对存在时会发生物理相互作用，这可能会使它们的清除更加困难。本研究采用质谱分析法，对体外和体内样本中这些相互作用的性质进行了探索。
我们将硅油（粘度为1000或5000厘沲）与PFCL[全氟正辛烷（PFO）或全氟三丁胺]在体外共同孵育6个月，并对PFCL进行电子轰击电离质谱分析（EIMS），以表征液相之间的相互作用。包装好的PFCL样本作为对照。我们还检查了在手术切除前已在人眼中留存数月的PFO的体内样本。
用于手术的包装全氟化碳液体均含有痕量的SiO，这可能是其制造过程的一种表现。此外，所有与SiO孵育的PFCL都显示出SiO分子碎片的污染更为明显。在两种液体都长时间存在的眼中，PFCL存在于SiO相中。对体内样本的EIMS分析表明，蛋白质包裹着PFCL液滴，形成类似胶束的结构。
医用级PFCL含有少量的SiO，并且随着时间的推移，PFCL会将少量的油溶解到溶液中。残留玻璃体替代物之间的相互作用可能具有临床意义。
