Gergely A, Szöllösi J, Falkai G, Resch B, Kovacs L, Huszar G
Department of Obstetrics and Gynecology, Albert Szent-Györgyi Medical University, Szeged, Hungary.
J Assist Reprod Genet. 1999 Jan;16(1):35-40. doi: 10.1023/a:1022545612784.
Our purpose was to measure sperm creatine phosphokinase (CK) activity, which reflects cytoplasmic retention in immature spermatozoa, in normospermic and oligozospermic Hungarian men.
A study of 109 randomly selected men in a university-based andrology laboratory was done.
CK activity differed between normospermic and oligozospermic men (0.21 +/- 0.02 vs. 1.19 +/- 0.15 CK IU/10(8) sperm; n = 56 and n = 53; mean +/- standard error of the mean, respectively). There was an inverse correlation between sperm concentration and CK activity (r = -0.70; n = 109). However, 28% of men in the range with less than 10 million sperm/ml had normal sperm CK activity (below the mean + 2 standard deviations of the group with greater than 30 x 10(6) sperm/ml), whereas 36% of men in the group with 20-30 million sperm/ml and 5% in the group with greater than 30 million sperm/ml had elevated CK activities, indicating that the incidence of mature and immature spermatozoa in specimens is independent from the sperm concentrations.
The improved facility of sperm CK activity measurements, compared with sperm concentrations, in the assessment of sperm maturity was confirmed in a Hungarian population. The CK measurements aid the selection of the most efficient treatment for couples with male-factor or unexplained infertility, particularly when considering the options of intrauterine insemination, varicocelectomy followed by a waiting period, or ovulation workup/induction in wives of men who are oligozospermic but may have fertile sperm.
我们的目的是测量正常精子数和少精子症匈牙利男性的精子肌酸磷酸激酶（CK）活性，该活性反映未成熟精子中的细胞质滞留情况。
在一所大学的男科实验室对109名随机选择的男性进行了研究。
正常精子数和少精子症男性的CK活性存在差异（分别为0.21±0.02与1.19±0.15 CK IU/10⁸精子；n = 56和n = 53；均值±均值标准误）。精子浓度与CK活性呈负相关（r = -0.70；n = 109）。然而，精子浓度低于1000万/ml范围内的男性中有28%的精子CK活性正常（低于精子浓度大于30×10⁶/ml组的均值+2个标准差），而精子浓度在2000万-3000万/ml组中的男性有36%以及精子浓度大于3000万/ml组中的男性有5%的CK活性升高，这表明标本中成熟和未成熟精子的发生率与精子浓度无关。
在匈牙利人群中证实，与精子浓度相比，测量精子CK活性在评估精子成熟度方面具有更好的效果。CK测量有助于为男性因素或不明原因不孕的夫妇选择最有效的治疗方法，特别是在考虑宫内授精、精索静脉曲张切除术后等待期或对少精子症但可能有可育精子的男性的妻子进行排卵检查/诱导等选择时。
