酵母中的S-(1,2-二羧乙基)谷胱甘肽：其合成酶的部分纯化

S-(1,2-Dicarboxyethyl)glutathione in yeast: partial purification of its synthesizing enzyme.

作者信息

Tsuboi S, Kiyono K, Ono B, Sakaue T, Ogata K

机构信息

Faculty of Pharmaceutical Sciences, Okayama University, Japan.

出版信息

Biol Pharm Bull. 1999 Jan;22(1):21-5. doi: 10.1248/bpb.22.21.

DOI:10.1248/bpb.22.21

PMID:9989656

Abstract

S-(1,2-Dicarboxyethyl)glutathione (DCE-GS) was found in Saccharomyces cerevisiae, but not in bacterial species nor in a unicellular alga (Acetabularia acetabulum). The enzyme that catalyzes condensation of L-malate and glutathione (GSH) to form DCE-GS was partially purified from baker's yeast. It had a molecular mass of 49 kDa and was monomeric and the Km values were 2.2 and 1.4 mM for L-malate and GSH, respectively. The enzyme had a pH optimum of 7.5. DCE-GS levels in yeast cells were significantly higher in aerobic cultures than in anaerobic ones. DCE-GS was synthesized in cells cultured between 20 and 35 degrees C.

摘要

在酿酒酵母中发现了S-(1,2-二羧乙基)谷胱甘肽（DCE-GS），但在细菌物种和单细胞藻类（伞藻）中未发现。催化L-苹果酸和谷胱甘肽（GSH）缩合形成DCE-GS的酶从面包酵母中得到了部分纯化。它的分子量为49 kDa，呈单体形式，L-苹果酸和GSH的Km值分别为2.2 mM和1.4 mM。该酶的最适pH值为7.5。酵母细胞中的DCE-GS水平在需氧培养物中明显高于厌氧培养物。DCE-GS在20至35摄氏度培养的细胞中合成。