Morris J R, Geyer P K, Wu C T
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA.
Genes Dev. 1999 Feb 1;13(3):253-8. doi: 10.1101/gad.13.3.253.
Transvection can cause the expression of a gene to be sensitive to the proximity of a homolog. It can account for many cases of intragenic complementation at the Drosophila yellow gene, where one mode of transvection involves the action of enhancers in trans on a promoter present on a separate chromosome. Our goal was to identify cis-acting elements that regulate the trans action of enhancers. Using gene replacement, we altered two core promoter elements at yellow and tested the resulting alleles for their ability to support transvection. We found that the TATA box and initiator element can regulate transvection.
异位效应可使基因表达对同源物的接近程度敏感。它可以解释果蝇黄色基因许多基因内互补的情况，其中一种异位效应模式涉及反式作用的增强子对存在于单独染色体上的启动子的作用。我们的目标是鉴定调控增强子反式作用的顺式作用元件。利用基因置换，我们改变了黄色基因的两个核心启动子元件，并测试所得等位基因支持异位效应的能力。我们发现TATA框和起始元件可以调控异位效应。
