大肠杆菌中通过RecBC途径进行的双链末端修复引发DNA复制。
Double-strand end repair via the RecBC pathway in Escherichia coli primes DNA replication.
作者信息
Kuzminov A, Stahl F W
机构信息
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229
出版信息
Genes Dev. 1999 Feb 1;13(3):345-56. doi: 10.1101/gad.13.3.345.
Abstract
To study the relationship between homologous recombination and DNA replication in Escherichia coli, we monitored the behavior of phage lambda chromosomes, repressed or not for lambda gene activities. Recombination in our system is stimulated both by DNA replication and by experimentally introduced double-strand ends, supporting the idea that DNA replication generates occasional double-strand ends. We report that the RecBC recombinational pathway of E. coli uses double-strand ends to prime DNA synthesis, implying a circular relationship between DNA replication and recombination and suggesting that the primary role of recombination is in the repair of disintegrated replication forks arising during vegetative reproduction.
摘要
为了研究大肠杆菌中同源重组与DNA复制之间的关系，我们监测了λ噬菌体染色体在λ基因活性被抑制或未被抑制时的行为。在我们的系统中，DNA复制和实验引入的双链末端均能刺激重组，这支持了DNA复制偶尔会产生双链末端的观点。我们报告称，大肠杆菌的RecBC重组途径利用双链末端启动DNA合成，这意味着DNA复制与重组之间存在循环关系，并表明重组的主要作用是修复营养繁殖过程中出现的解体复制叉。
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