A kinetic nephelometric method for the assay of serum C3 and C4.

A two-point, fixed interval kinetic, nephelometric method for the immunoassay of serum C3 and serum C4 using a laser-modified, centrifugal fast analyzer is described. The procedure shows a precision of 2.45 percent (coefficient of variation, C.V.) for C3 at a mean concentration of 151 mg per dl and 3.65 percent (C.V.) for C4 at a mean concentration of 26.6 mg per dl. The kinetic method was found to give a satisfactory correlation with a continuous flow immunonephelometric procedure.