Inoue Y, Furuta S, Oku T, Shibata K
Biochim Biophys Acta. 1976 Dec 6;449(3):357-67. doi: 10.1016/0005-2728(76)90147-x.
Thermoluminescence profiles of spruce leaves grown under various light or dark conditions were measured after excitation at a low temperature (-70 to -20 degrees C) by 1-min illumination with red light, and the following results were obtained. Mature spruce leaves showed five thermoluminescence bands at -30, -5, +20, +40 (or +35) and +70 degrees C (denoted as Zv, A, B1, B2 and C bands, respectively), but dark-grown spruce leaves with a similar chlorophyll content showed only two bands, at -30 and +70 degrees C (the Zv and C bands) and were devoid of the three other bands (the A, B1, and B2 bands). On exposure of the dark-grown leaves to continuous red light, the A, B1and B2 bands were rapidly developed, and the development was accompanied by enhancement of delayed emission, fluorescence variation and the Hill activity (photoreduction of 2,6-dichlorophenolindophenol with water as electron donor). It was demonstrated that the dark-grown spruce leaves are devoid of the water-splitting system in Photosystem II, and that the latent water-splitting activity is rapidly photoactivated by exposure of the leaves to continuous red light. These results on the gymnosperm spruce leaves, in which greening proceeds in complete darkness, being independent of the development of the water-splitting system in light, were discussed in relation to previous observations on angiosperm leaves, in which both greening and the activity generation proceed in the light.
在低温（-70至-20摄氏度）下用红光照射1分钟激发后，测量了在各种光照或黑暗条件下生长的云杉叶片的热释光曲线，得到了以下结果。成熟的云杉叶片在-30、-5、+20、+40（或+35）和+70摄氏度处显示出五个热释光带（分别表示为Zv、A、B1、B2和C带），但叶绿素含量相似的黑暗中生长的云杉叶片仅在-30和+70摄氏度处显示出两个带（Zv和C带），并且没有其他三个带（A、B1和B2带）。将黑暗中生长的叶片暴露于连续红光下，A、B1和B2带迅速形成，并且这种形成伴随着延迟发光增强、荧光变化和希尔活性（以水作为电子供体对2,6-二氯酚靛酚进行光还原）的增强。结果表明，黑暗中生长的云杉叶片在光系统II中没有水裂解系统，并且叶片暴露于连续红光下可使潜在的水裂解活性迅速光激活。这些关于裸子植物云杉叶片的结果，其在完全黑暗中进行绿化，与光下水裂解系统的发育无关，结合先前对被子植物叶片的观察进行了讨论，被子植物叶片的绿化和活性产生均在光照下进行。
