Grattard F, Gaudin O G, Pozzetto B, Ros A, Mbida A D
Department of Bacteriology-Virology, Faculty of Medicine, Jacques Lisfranc, University of Saint Etienne, France.
Eur J Clin Microbiol Infect Dis. 1993 Jan;12(1):57-61. doi: 10.1007/BF01997061.
The spread in Europe of a single multiresistant strain of Pseudomonas aeruginosa serotype O12 has been suggested. This bacterium was responsible for a nosocomial outbreak in our hospital in 1988-1989. Three different epidemiological methods were used to analyze 30 strains isolated during five consecutive years. Protein profile analysis and chromosomal DNA fingerprinting with four different enzymes revealed closely related patterns. rRNA gene restriction fragment length analysis performed with a digoxigenin-labelled probe showed identical hybridization patterns with four to six bands according to the endonuclease used. Combination of the three typing methods showed genotypic homogeneity of these Pseudomonas aeruginosa O12 strains, despite a relative increase in their antibiotic resistance.
有人提出，欧洲已出现单一多重耐药性铜绿假单胞菌血清型O12菌株的传播。该细菌在1988 - 1989年期间导致了我院的一次医院感染暴发。我们使用三种不同的流行病学方法对连续五年分离出的30株菌株进行了分析。蛋白质谱分析以及用四种不同酶进行的染色体DNA指纹图谱分析显示出密切相关的模式。使用地高辛标记探针进行的rRNA基因限制性片段长度分析表明，根据所使用的内切酶不同，会出现具有四到六条带的相同杂交模式。尽管这些铜绿假单胞菌O12菌株的抗生素耐药性有所相对增加，但三种分型方法的结合显示出这些菌株的基因型同质性。
