Reid G A, Gordon E H
Institute of Cell and Molecular Biology, University of Edinburgh, UK.
Int J Syst Bacteriol. 1999 Jan;49 Pt 1:189-91. doi: 10.1099/00207713-49-1-189.
Dissimilatory Fe(III) reduction by Shewanella putrefaciens and related species has generated considerable interest in biochemical characterization of the pathways for anaerobic electron transfer in this organism. Two strains, MR-1 and NCIMB 400, have been extensively used, and several respiratory enzymes have been isolated from each. It has become apparent that significant sequence differences exist between homologous proteins from these strains. The 16S rRNA from NCIMB 400 was sequenced and compared to the sequences from MR-1 and other Shewanella strains. The results indicate that NCIMB 400 is significantly more closely related to the newly identified Shewanella frigidimarina than to the S. putrefaciens type strain. It is therefore proposed that NCIMB 400 should be reclassified as S. frigidimarina.
腐败希瓦氏菌及相关菌种异化铁（III）还原作用引发了人们对该生物体厌氧电子传递途径生化特性的浓厚兴趣。MR-1和NCIMB 400这两个菌株得到了广泛应用，并且已从每个菌株中分离出了几种呼吸酶。很明显，这些菌株同源蛋白之间存在显著的序列差异。对NCIMB 400的16S rRNA进行了测序，并与MR-1和其他希瓦氏菌菌株的序列进行了比较。结果表明，NCIMB 400与新鉴定的嗜冷希瓦氏菌的亲缘关系明显比与腐败希瓦氏菌模式菌株的亲缘关系更近。因此，建议将NCIMB 400重新分类为嗜冷希瓦氏菌。
