Rediske A M, Rapoport N, Pitt W G
Brigham Young University, Provo, UT 84602, USA.
Lett Appl Microbiol. 1999 Jan;28(1):81-4. doi: 10.1046/j.1365-2672.1999.00461.x.
The effect of erythromycin on planktonic cultures of Psedomonas aeruginosa, with and without application of 70 kHz ultrasound, was studied. Ultrasound was applied at levels that had no inhibitory effect on cultures of Ps. aeruginosa. Ultrasound in combination with erythromycin reduced the viability of Ps. aeruginosa by 1-2 orders of magnitude compared with antibiotic alone, even at concentrations below the minimum inhibitory concentration (MIC). Electron-spin resonance studies suggest that ultrasound induces uptake of antibiotic by perturbing or stressing the membrane. This application of ultrasound may be useful for expanding the number of drugs available for treating localized infections by rendering bacteria susceptible to normally ineffective antibiotics.
研究了红霉素对铜绿假单胞菌浮游培养物的影响，包括施加和不施加70kHz超声的情况。施加的超声水平对铜绿假单胞菌培养物没有抑制作用。与单独使用抗生素相比，超声与红霉素联合使用可使铜绿假单胞菌的活力降低1-2个数量级，即使在低于最低抑菌浓度（MIC）的浓度下也是如此。电子自旋共振研究表明，超声通过扰动或使膜应激来诱导抗生素的摄取。这种超声应用可能有助于增加可用于治疗局部感染的药物数量，使细菌对通常无效的抗生素敏感。
