Ravid R, Eckstein B
J Endocrinol. 1976 Dec;71(3):299-304. doi: 10.1677/joe.0.0710299.
The conjugation of 5alpha-androstane-3alpha,17beta-diol (3alpha-A) and its 3beta epimer (3beta-A) was determined in the peripheral blood of immature female rats. About two thirds of these steroids were present in blood as sulphates and one third as glucuronides; no free steroids were detected. Administration of 3beta-A sulphate (25mug/100 g body weight/day) and of 3alpha-A sulphate (50 mug/100 g/day) from day 21 of life until the day of vaginal opening, advanced the day of the first ovulation. Administration of the 3beta-A sulphate did not induce precocious vaginal opening whereas the free alcohol was active in this respect. Implantation of 3beta-A sulphate, but not of the 3alpha epimer, into the basal medial hypothalamus resulted in the dealth of all animals within 24 h.
在未成熟雌性大鼠的外周血中测定了5α-雄烷-3α,17β-二醇（3α-A）及其3β差向异构体（3β-A）的结合情况。这些类固醇约三分之二以硫酸盐形式存在于血液中，三分之一以葡萄糖醛酸苷形式存在；未检测到游离类固醇。从出生后第21天到阴道开口之日，每天给予3β-A硫酸盐（25μg/100g体重）和3α-A硫酸盐（50μg/100g），可使首次排卵的日期提前。给予3β-A硫酸盐不会诱导早熟的阴道开口，而游离醇在这方面具有活性。将3β-A硫酸盐而非3α差向异构体植入基底内侧下丘脑会导致所有动物在24小时内死亡。
