Jessup J M, Cohen M H, Tomaszewski M M, Felix E L
J Natl Cancer Inst. 1976 Nov;57(5):1077-84. doi: 10.1093/jnci/57.5.1077.
In the murine model presented for tumor-associated immune suppression, normal BALB/c mice displayed significant foodpad swelling when sensitized on the flank with 2 mg dinitrochlorobenzene (DNCB) dissolved in dimethyl sulfoxide and challenged in a footpad with 0.05 mg DNCB 10 days later. This reaction in challenged footpads seemed to be a classic delayed hypersensitivity reaction, since it took 24 hours to develop and included an extensive mononuclear infiltrate. The reaction was transmissible from sensitized to normal mice by the transfer of lymphoid cells but not to serum. When sensitized 10 days after tumor inoculation, mice bearing either an allogeneic melanoma or a syngeneic lymphoma or fibrosarcoma did not demonstrate delayed hypersensitivity to DNCB.
在用于肿瘤相关免疫抑制的小鼠模型中，正常BALB/c小鼠在侧腹用溶解于二甲基亚砜的2mg二硝基氯苯（DNCB）致敏，并于10天后在足垫用0.05mg DNCB激发时，足垫出现明显肿胀。激发足垫的这种反应似乎是典型的迟发型超敏反应，因为它需要24小时才会出现，并且有大量单核细胞浸润。该反应可通过淋巴细胞转移从致敏小鼠传递给正常小鼠，但不能通过血清传递。当在肿瘤接种10天后致敏时，携带同种异体黑色素瘤或同基因淋巴瘤或纤维肉瘤的小鼠对DNCB未表现出迟发型超敏反应。
