在巴西南部人群的食管鳞状细胞癌中未发现 HPV DNA。
No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil.
机构信息
Luís Carlos Moreira Antunes, João Carlos Prolla, Antonio de Barros Lopes, Renato Borges Fagundes, Programa de Pós-Graduação: Ciências em Gastroenterologia e Hepatologia - Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 90035-003, Brasil.
出版信息
World J Gastroenterol. 2013 Oct 21;19(39):6598-603. doi: 10.3748/wjg.v19.i39.6598.
AIM
To investigate the association between human papillomavirus (HPV) and esophageal squamous cell carcinoma (ESCC) in southern Brazil.
METHODS
We studied 189 esophageal samples from 125 patients from three different groups: (1) 102 biopsies from 51 patients with ESCC, with one sample from the tumor and another from normal esophageal mucosa distant from the tumor; (2) 50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma (HNSCC); and (3) 37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients, not exposed to smoking or alcohol consumption. Nested-polymerase chain reaction (PCR) with the MY09/11 and GP5/6 L1 primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks. All PCR reactions were performed with a positive control (cervicovaginal samples), with a negative control (Human Genomic DNA) and with a blank reaction containing all reagents except DNA. We took extreme care to prevent DNA contamination in sample collection, processing, and testing.
RESULTS
The histological biopsies confirmed the diagnosis of ESCC in 52 samples (51 from ESCC group and 1 from the HNSCC group) and classified as well differentiated (12/52, 23.1%), moderately differentiated (27/52, 51.9%) or poorly differentiated (7/52, 13.5%). One hundred twenty-eight esophageal biopsies were considered normal (51 from the ESCC group, 42 from the HNSCC group and 35 from dyspeptic patients). Nine had esophagitis (7 from the HNSCC and 2 from dyspeptic patients). Of a total of 189 samples, only 6 samples had insufficient material for PCR analysis: 1 from mucosa distant from the tumor in a patient with ESCC, 3 from patients with HNSCC and 2 from patients without cancer. In 183 samples (96.8%) GAPDH, G3PDH and/or β-globin were amplified, thus indicating the adequacy of the DNA in those samples. HPV DNA was negative in all the 183 samples tested: 52 with ESCC, 9 with esophagitis and 122 with normal esophageal mucosa.
CONCLUSION
There was no evidence of HPV infection in different ESCC from southern Brazil.
目的
研究巴西南部人乳头瘤病毒（HPV）与食管鳞状细胞癌（ESCC）的关系。
方法
我们研究了来自三个不同组的 189 个食管样本：（1）102 个来自 51 例 ESCC 患者的活检，其中一个来自肿瘤，另一个来自肿瘤远处的正常食管黏膜；（2）50 个来自 37 例头颈部鳞状细胞癌（HNSCC）患者的食管活检；（3）37 个来自 37 例消化不良患者的正常外观食管黏膜活检，这些患者无吸烟或饮酒史。使用 MY09/11 和 GP5/6 L1 引物的巢式聚合酶链反应（PCR）检测固定在福尔马林和石蜡块中的样本中的 HPV L1。所有 PCR 反应均使用阳性对照（宫颈阴道样本）、阴性对照（人基因组 DNA）和不含 DNA 的空白反应进行。我们非常小心地防止在样本采集、处理和检测过程中发生 DNA 污染。
结果
组织学活检证实 52 个样本（ESCC 组 51 个，HNSCC 组 1 个）诊断为 ESCC，并分为高分化（12/52，23.1%）、中分化（27/52，51.9%）或低分化（7/52，13.5%）。128 个食管活检被认为是正常的（ESCC 组 51 个，HNSCC 组 42 个，消化不良患者 35 个）。9 个有食管炎（7 个来自 HNSCC，2 个来自消化不良患者）。在总共 189 个样本中，只有 6 个样本的 PCR 分析材料不足：1 个来自 ESCC 患者肿瘤远处的黏膜，3 个来自 HNSCC 患者，2 个来自无癌症患者。在 183 个样本（96.8%）中扩增了 GAPDH、G3PDH 和/或 β-珠蛋白，因此表明这些样本中的 DNA 充足。在所有 183 个测试样本中均未检测到 HPV DNA：52 个有 ESCC，9 个有食管炎，122 个有正常食管黏膜。
结论
巴西南部不同 ESCC 中没有 HPV 感染的证据。
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