放线菌科及相关细菌的快速鉴定。
Rapid identification of Actinomycetaceae and related bacteria.
作者信息
Kilian M
出版信息
J Clin Microbiol. 1978 Aug;8(2):127-33. doi: 10.1128/jcm.8.2.127-133.1978.
Abstract
Identification of new isolates belonging to the family Actinomycetaceae requires extensive numbers of biochemical tests, supplemented with gas-liquid chromatography determination of fermentation end products and, often, analysis of cell wall composition. This paper describes the results of the testing of 162 strains of Actinomycetaceae and related taxa for 20 different enzymatic activities including phosphatases, esterases, aminopeptidases, and glycosidases. The results of all tests were read after 4 h of incubation. The results obtained in the study provide significant new information on the biochemical properties of these groups of bacteria. An identification scheme based upon 13 selected tests, which allow the identification of these groups of bacteria within 4 h, is proposed.
摘要
鉴定属于放线菌科的新分离株需要进行大量的生化试验，并辅以发酵终产物的气液色谱测定，而且通常还需要分析细胞壁成分。本文描述了对162株放线菌科及相关分类群菌株进行20种不同酶活性测试的结果，这些酶活性包括磷酸酶、酯酶、氨肽酶和糖苷酶。所有测试结果在培养4小时后读取。该研究获得的结果为这些细菌群体的生化特性提供了重要的新信息。本文提出了一种基于13项选定测试的鉴定方案，该方案可在4小时内鉴定出这些细菌群体。
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