Odani H, Shinzato T, Matsumoto Y, Usami J, Maeda K
Department of Internal Medicine, Nagoya University Daiko Medical Center, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, 461-0047, Japan.
Biochem Biophys Res Commun. 1999 Mar 5;256(1):89-93. doi: 10.1006/bbrc.1999.0221.
Methylglyoxal (MGO), glypxal (GO) and 3-deoxyglucosone (3-DG) are reactive alpha,beta-dicarbonyl intermediates in advanced Maillard reaction, which form advanced glycation and oxidation end products (AGEs) by reaction with both lysine and arginine residues in protein. We measured these three dicarbonyl compound levels in human plasma to estimate the relationship between accumulation of alpha, beta-dicarbonyl compounds and AGE formation reactions in uremia and diabetes in human plasma by a highly selective and specific assay, electrospray ionization liquid chromatography mass spectrometry (ESI/LC/MS). We show that 3-DG and MGO levels are significantly higher in uremia and diabetes compared with age-matched healthy controls. Only the GO level in uremic plasma is significantly higher compared to diabetes and healthy controls. In both diabetic and uremic patients, these dicarbonyl compounds promote AGE accumulation in vivo, and especially in uremic patients, increased accumulation of GO could result from accelerating oxidative stress.
甲基乙二醛（MGO）、乙二醛（GO）和3-脱氧葡萄糖醛酮（3-DG）是晚期美拉德反应中具有反应活性的α,β-二羰基中间体，它们通过与蛋白质中的赖氨酸和精氨酸残基反应形成晚期糖基化终产物（AGEs）。我们通过高选择性和特异性的电喷雾电离液相色谱质谱分析法（ESI/LC/MS）测定了人血浆中这三种二羰基化合物的水平，以评估α,β-二羰基化合物的积累与人血浆中尿毒症和糖尿病患者体内AGE形成反应之间的关系。我们发现，与年龄匹配的健康对照组相比，尿毒症和糖尿病患者血浆中的3-DG和MGO水平显著更高。与糖尿病和健康对照组相比，仅尿毒症患者血浆中的GO水平显著更高。在糖尿病和尿毒症患者体内，这些二羰基化合物均会促进AGE的积累，尤其是在尿毒症患者中，GO积累增加可能是由于氧化应激加速所致。
