Bauer J
Max-Planck-Institut für Biochemie, Martinsried, Germany.
J Chromatogr B Biomed Sci Appl. 1999 Feb 5;722(1-2):55-69. doi: 10.1016/s0378-4347(98)00308-9.
Cell separation by counterflow centrifugal elutriation (CCE) or free flow electrophoresis (FFE) is performed at lower frequency than cell cloning and antibody-dependent, magnetic or fluorescence-activated cell sorting. Nevertheless, numerous recent publications confirmed that these physical cell separation methods that do not include cell labeling or cell transformation steps, may be most useful for some applications. CCE and FFE have proved to be valuable tools, if homogeneous populations of normal healthy untransformed cells are required for answering scientific questions or for clinical transplantation and cells cannot be labeled by antibodies, because suitable antibodies are not available or because antibody binding to a cell surface would induce the cell reaction which should be investigated on purified cells or because antibodies bound to the surface hamper the use of the isolated cells. In addition, the methods are helpful for studying the biological reasons for, or effects of, changes in cell size and cellular negative surface charge density. Although the value of the methods was confirmed in recent years by a considerable number of important scientific results, activities to further develop and improve the instruments have, unfortunately, declined.
通过逆流离心淘析（CCE）或自由流动电泳（FFE）进行细胞分离的频率低于细胞克隆以及抗体依赖、磁性或荧光激活细胞分选。然而，最近大量的出版物证实，这些不包括细胞标记或细胞转化步骤的物理细胞分离方法，可能对某些应用最为有用。如果回答科学问题或进行临床移植需要正常健康未转化细胞的同质群体，并且细胞不能用抗体标记，例如因为没有合适的抗体，或者因为抗体与细胞表面结合会诱导细胞反应（而这正是需要在纯化细胞上进行研究的），或者因为结合在表面的抗体妨碍了分离细胞的使用，那么CCE和FFE已被证明是有价值的工具。此外，这些方法有助于研究细胞大小变化和细胞表面负电荷密度变化的生物学原因或影响。尽管近年来大量重要的科学成果证实了这些方法的价值，但遗憾的是，进一步开发和改进仪器的活动却减少了。
