Ogiso M, Hoshi M, Nishigori H
Cell and Information, PRESTO, Japan Science and Technology Corporation (JST), Japan.
Exp Eye Res. 1999 Feb;68(2):229-36. doi: 10.1006/exer.1998.0598.
Administration of glucocorticoids induces transient cataract in 15-day-old chick embryos within 48 hr, and the opaque lens again becomes clear within the subsequent 48 hr. Oxidative stress is likely to be involved in the process of cataract formation, resulting in the appearance of numerous vacuoles around the perinuclear region. Chick lens contained low amounts of glycosphingolipids, which mainly consists of GM3, GD3, sialyl-LewisX gangliosides and glucosylceramide. Most lens gangliosides were immunohistochemically detected in lens epithelia, annular pads and developing fibers, but not in perinuclear and nuclear regions. Since cell surface gangliosides, for example GM3 and sialyl-LewisX gangliosides, are involved in cell adhesion, weak cell-to-cell interactions in the perinuclear and nuclear regions may allow vacuole formation in steroid-induced cataractogenesis.
给予糖皮质激素会在48小时内诱导15日龄鸡胚出现短暂性白内障，并且在随后的48小时内晶状体混浊会再次变清。氧化应激可能参与了白内障形成过程，导致核周区域周围出现大量空泡。鸡晶状体含有少量糖鞘脂，主要由GM3、GD3、唾液酸化路易斯X神经节苷脂和葡萄糖神经酰胺组成。大多数晶状体神经节苷脂通过免疫组织化学方法在晶状体上皮、环状垫和发育中的纤维中检测到，但在核周和核区域未检测到。由于细胞表面神经节苷脂，例如GM3和唾液酸化路易斯X神经节苷脂，参与细胞黏附，核周和核区域中细胞间相互作用减弱可能会在类固醇诱导的白内障形成过程中导致空泡形成。
