Nishigori H, Hayashi R, Lee J W, Maruyama K, Iwatsuru M
Exp Eye Res. 1985 Mar;40(3):445-51. doi: 10.1016/0014-4835(85)90157-5.
Glucocorticoid administration to developing chick embryos is known to promote cataract formation with a decreasing level of glutathione in the lens. To gain further understanding of this process, the level of ascorbic acid, a biological antioxidant, in the lenses was measured during the course of glucocorticoid treatment. When 0.25 mumol of hydrocortisone hemisuccinate sodium (HC) were administered to 15-day-old chick embryos, the level of ascorbic acid in the lens began to decline after 30 hr and became around 40% of the control value at 48 hr after HC treatment. At this time about 90% of the lenses showed opacity in the nuclear region. However, the level of ascorbic acid in the cataractous lens recovered to the control level at 96 hr, a time when the lens has recovered from cataract formation. A triple application of ascorbic acid (20 mumol/egg) at 3, 10 and 20 hr after HC treatment significantly prevented lens opacification. The administration of ascorbic acid prevented the decline of ascorbic acid content and partially that of glutathione content in the lens caused by HC.
已知对发育中的鸡胚施用糖皮质激素会促进白内障形成，同时晶状体中谷胱甘肽水平会降低。为了进一步了解这一过程，在糖皮质激素治疗过程中测量了晶状体中生物抗氧化剂抗坏血酸的水平。当向15日龄鸡胚施用0.25微摩尔半琥珀酸氢化可的松钠（HC）时，晶状体中抗坏血酸水平在30小时后开始下降，在HC处理后48小时降至对照值的约40%。此时约90%的晶状体在核区域出现混浊。然而，白内障晶状体中的抗坏血酸水平在96小时恢复到对照水平，此时晶状体已从白内障形成中恢复。在HC处理后3、10和20小时三次施用抗坏血酸（20微摩尔/蛋）可显著预防晶状体混浊。施用抗坏血酸可防止晶状体中抗坏血酸含量的下降，并部分防止HC引起的谷胱甘肽含量的下降。
