Silversmith R E, Bourret R B
Dept of Microbiology and Immunology, University of North Carolina, Chapel Hill 27599-7290, USA.
Trends Microbiol. 1999 Jan;7(1):16-22. doi: 10.1016/s0966-842x(98)01409-7.
In Escherichia coli chemotaxis, the switch from counterclockwise to clockwise rotation of the flagella occurs as a result of binding of the phosphorylated CheY protein to the base of the flagellum. Analysis of CheY variants has provided a picture of the surface of CheY that undergoes conformational shifts, as a result of phosphorylation, to interact directly with the flagellum. Whether phospho-CheY binding and flagellar switching are sequential steps or can occur in a concerted fashion has yet to be determined.
在大肠杆菌的趋化作用中，鞭毛从逆时针旋转转变为顺时针旋转是磷酸化的CheY蛋白与鞭毛基部结合的结果。对CheY变体的分析揭示了CheY的表面情况，该表面由于磷酸化而发生构象变化，从而直接与鞭毛相互作用。磷酸化的CheY结合和鞭毛切换是相继发生的步骤还是可以协同发生，尚待确定。
