Wilkins P P, Allan J C, Verastegui M, Acosta M, Eason A G, Garcia H H, Gonzalez A E, Gilman R H, Tsang V C
Immunology Branch, Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.
Am J Trop Med Hyg. 1999 Feb;60(2):199-204. doi: 10.4269/ajtmh.1999.60.199.
We developed a serologic assay to identify adult Taenia solium tapeworm carriers using excretory/secretory (TSES) antigens collected from in vitro cultured T. solium tapeworms. To identify taeniasis-specific antigens we used an immunoblot assay with serum samples from T. solium tapeworm carriers and cysticercosis patients. Antigens were identified that reacted with antibodies present in serum samples from taeniasis cases and not with those from cysticercosis patients. Using serum samples collected from persons with confirmed T. solium tapeworm infections, the test was determined to be 95% (69 of 73) sensitive. Serum samples (n = 193) from persons with other parasitic infections, including T. saginata tapeworm infections, do not contain cross-reacting antibodies to TSES, indicating that the assay is 100% specific. These data suggest that the immunoblot assay using TSES antigens can be used to identify persons with current or recent T. solium tapeworm infections and provides a new, important tool for epidemiologic purposes, including control and prevention strategies.
我们开发了一种血清学检测方法，用于使用从体外培养的猪带绦虫收集的排泄/分泌（TSES）抗原鉴定成年猪带绦虫携带者。为了鉴定绦虫病特异性抗原，我们对猪带绦虫携带者和囊尾蚴病患者的血清样本进行了免疫印迹分析。鉴定出与绦虫病病例血清样本中存在的抗体发生反应但不与囊尾蚴病患者血清样本中的抗体发生反应的抗原。使用从确诊为猪带绦虫感染的人收集的血清样本，该检测方法的灵敏度为95%（73例中的69例）。来自其他寄生虫感染患者（包括牛带绦虫感染）的血清样本（n = 193）不含与TSES发生交叉反应的抗体，表明该检测方法具有100%的特异性。这些数据表明，使用TSES抗原的免疫印迹分析可用于鉴定当前或近期感染猪带绦虫的人，并为包括控制和预防策略在内的流行病学目的提供了一种新的重要工具。
