Myrta A, Potere O, Boscia D, Candresse T, Cambra M, Savino V
Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi, Bari, Italy.
Acta Virol. 1998 Sep;42(4):248-50.
Plum pox virus (PPV) isolates are grouped into three clusters differentiated by biological, serological, molecular and epidemiological characteristics: Marcus (M), Dideron (D) and Cherry (C). The El Amar (EA) isolate that does not fit any of the above groups is also known. Monoclonal antibodies (MAbs) that specifically recognize M, D, and C strains of PPV are already available. To complete the set of PPV strain-specific serological reagents, MAbs against the EA isolate were raised by immunizing BALB/c mice and fusing their spleen cells with NS0/1 myeloma cells. After a preliminary characterization by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), 1 of 13 selected MAbs proved to be EA strain-specific. This MAb (EA24) reacted equally well with a homologous antigen and several PPV isolates from Egyptian apricot trees, supporting the hypothesis of an additional specific PPV group. MAb EA24 did not react either with about a hundred PPV isolates belonging to the D and M groups or with PPV-SwC and PPV-SoC isolates belonging to the C group. The strain specificity of MAb EA24 was confirmed by Western blot analysis and immunoelectron microscopy. We conclude that there is now available a set of MAbs which are highly specific to the four currently known groups of PPV strains.
李痘病毒（PPV）分离株可分为三个簇，通过生物学、血清学、分子和流行病学特征进行区分：马库斯（M）、迪德龙（D）和樱桃（C）。也已知不符合上述任何一组的埃尔阿马尔（EA）分离株。已经有能特异性识别PPV的M、D和C株的单克隆抗体（MAb）。为了完善PPV株特异性血清学试剂，通过免疫BALB/c小鼠并将其脾细胞与NS0/1骨髓瘤细胞融合，制备了针对EA分离株的单克隆抗体。通过双抗体夹心酶联免疫吸附测定（DAS-ELISA）进行初步鉴定后，13株筛选出的单克隆抗体中有1株被证明是EA株特异性的。这种单克隆抗体（EA24）与同源抗原以及来自埃及杏树的几种PPV分离株反应良好，支持了存在另一个特定PPV组的假设。单克隆抗体EA24与大约100株属于D组和M组的PPV分离株以及属于C组的PPV-SwC和PPV-SoC分离株均无反应。通过蛋白质印迹分析和免疫电子显微镜证实了单克隆抗体EA24的株特异性。我们得出结论，现在有一组对目前已知的四类PPV株具有高度特异性的单克隆抗体。
