Molecular variability of Czech plum pox virus isolates.

Seventy-two Czech plum pox virus (PPV) isolates from different hosts were tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). In addition, the coat protein mobility and the RsaI restriction fragment length polymorphism (RFLP) pattern of reverse transcription-polymerase chain reaction-(RT-PCR) amplified coat protein (CP) gene fragment of the isolates were analysed. Both PPV-D and PPV-M serotypes were found in the Czech Republic. The results obtained by these different methods were in accord with exception of a few cases probably caused by mutation or recombination.