Navrátil M, Simonová V, Fialová R, Válová P
Department of Cell Biology and Genetics, Faculty of Science, Palacky University, Olomouc, Czech Republic.
Acta Virol. 1998 Sep;42(4):254-6.
Seventy-two Czech plum pox virus (PPV) isolates from different hosts were tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). In addition, the coat protein mobility and the RsaI restriction fragment length polymorphism (RFLP) pattern of reverse transcription-polymerase chain reaction-(RT-PCR) amplified coat protein (CP) gene fragment of the isolates were analysed. Both PPV-D and PPV-M serotypes were found in the Czech Republic. The results obtained by these different methods were in accord with exception of a few cases probably caused by mutation or recombination.
通过双抗体夹心酶联免疫吸附测定（DAS-ELISA）对来自不同宿主的72株捷克李痘病毒（PPV）分离株进行了检测。此外，还分析了这些分离株的外壳蛋白迁移率以及逆转录-聚合酶链反应（RT-PCR）扩增的外壳蛋白（CP）基因片段的RsaI限制性片段长度多态性（RFLP）模式。在捷克共和国发现了PPV-D和PPV-M两种血清型。除了少数可能由突变或重组引起的情况外，这些不同方法获得的结果是一致的。
