Myrta A, Di Terlizzi B, Boscia D, Caglayan K, Gavriel I, Ghanem G, Varveri C, Savino V
Ministry of Agriculture and Food, Tirana, Albania.
Acta Virol. 1998 Sep;42(4):251-3.
Plum pox virus (PPV) is a major threat to the expanding Mediterranean stone fruit industry. In order to control the plum pox disease it is of utmost importance to detect early PPV foci and to identify the PPV isolates involved. A survey was therefore carried out in Albania, Cyprus, Egypt, Greece, Italy and Turkey by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with the following monoclonal antibodies (MAbs): 5B (universal), 4DG5 (PPV-D-specific), AL (PPV-M-specific), TUV and AC (PPV-C-specific), and EA24 (PPV-El Amar-specific). A hundred and seventy Mediterranean PPV isolates were tested for strain type. PPV-M was detected in Albania, Cyprus, Greece, Italy, and Turkey; PPV-D was detected in Albania and Italy, whereas samples with natural mixtures of both strains were found in a couple of orchards in Albania. Seven PPV isolates from apricots in two Egyptian localities were recognized only by MAb EA24. In conclusion, DAS-ELISA with a combination of the universal MAb5B and the MAbs specific to the four PPV serotypes currently known (M, D, C and El Amar) is an efficient tool for a simple, sensitive and routine detection of PPV and discrimination of its serotypes.
李痘病毒（PPV）对不断发展的地中海核果产业构成重大威胁。为了控制李痘病，尽早发现PPV疫点并鉴定相关的PPV分离株至关重要。因此，在阿尔巴尼亚、塞浦路斯、埃及、希腊、意大利和土耳其开展了一项调查，采用双抗体夹心酶联免疫吸附测定（DAS-ELISA），使用以下单克隆抗体（MAb）：5B（通用型）、4DG5（PPV-D特异性）、AL（PPV-M特异性）、TUV和AC（PPV-C特异性），以及EA24（PPV-埃尔阿马尔特异性）。对170个地中海PPV分离株进行了毒株类型检测。在阿尔巴尼亚、塞浦路斯、希腊、意大利和土耳其检测到PPV-M；在阿尔巴尼亚和意大利检测到PPV-D，而在阿尔巴尼亚的几个果园中发现了两种毒株自然混合的样本。来自埃及两个地区杏树的7个PPV分离株仅能被单克隆抗体EA24识别。总之，将通用单克隆抗体5B与目前已知的四种PPV血清型（M、D、C和埃尔阿马尔）特异性单克隆抗体结合使用的DAS-ELISA，是一种简单、灵敏且常规检测PPV及其血清型鉴别的有效工具。
