Glasa M, Matisová J, Kúdela O
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Acta Virol. 1998 Sep;42(4):226-9.
Plum pox virus (PPV) isolates from stone-fruit trees (plums, myrobalans, apricots and peaches) from orchards and gardens were characterized. To characterize their biological properties, several PPV isolates were transmitted by chip budding to GF 305 seedlings and mechanically to selected herbaceous test plants. The isolates differed in severity of infection, host range and symptomatology. A subgroup differentiation of 43 isolates from 22 localities of western and middle Slovakia was accomplished using reverse transcription-polymerase chain reaction (RT-PCR), immunocapture RT-PCR (IC-RT-PCR) and restriction analysis. These assays confirmed the presence of isolates belonging to PPV-M and PPV-D subgroups. PPV-M and PPV-D isolates were almost equally represented in tested samples. Tests of subgroup variability of PPV isolates from infected tolerant plum cultivars showed great predominance of PPV-M isolates.
对从果园和花园的核果类果树（李子、樱桃李、杏子和桃子）中分离出的李痘病毒（PPV）分离株进行了特性鉴定。为了鉴定其生物学特性，通过芽接将几种PPV分离株接种到GF 305幼苗上，并通过机械接种到选定的草本试验植物上。这些分离株在感染严重程度、寄主范围和症状表现方面存在差异。利用逆转录-聚合酶链反应（RT-PCR）、免疫捕获RT-PCR（IC-RT-PCR）和限制性分析，对斯洛伐克西部和中部22个地区的43个分离株进行了亚组区分。这些检测证实了PPV-M和PPV-D亚组分离株的存在。在测试样本中，PPV-M和PPV-D分离株的比例几乎相等。对感染了耐病李品种的PPV分离株进行亚组变异性测试，结果显示PPV-M分离株占主导地位。
