Kúdela O, Glasa M, Fuchs E, Kúdelová M
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Acta Virol. 1998 Apr;42(2):71-4.
Leaf tissues of stone fruit trees (plum, apricot, peach and myrobalan) carrying symptoms of plum pox virus (PPV) infection and of peach GF 305 seedlings and Nicotiana benthamiana infected experimentally with PPV were assayed for PPV by polymerase chain reaction (PCR). The expected 243 bp PCR products were subjected to restriction fragment length polymorphism (RFLP) analysis with restriction endonucleases AluI and RsaI. All of the PCR products contained the AluI site. The RsaI restriction profiles of the PCR products demonstrated the prevalence of PPV-M subgroup in the tested samples from western Slovakia.
对携带李痘病毒（PPV）感染症状的核果类果树（李子、杏子、桃子和樱桃李）的叶片组织、经PPV实验感染的桃GF 305幼苗和本氏烟草进行聚合酶链反应（PCR）检测以确定是否存在PPV。预期的243 bp PCR产物用限制性内切酶AluI和RsaI进行限制性片段长度多态性（RFLP）分析。所有PCR产物都含有AluI位点。PCR产物的RsaI限制性图谱表明斯洛伐克西部测试样品中PPV-M亚组占主导。
