Polák J
Research Institute of Crop Production, Division of Phytomedicine, Prague, Czech Republic.
Acta Virol. 1998 Sep;42(4):264-7.
The relative concentration of plum pox virus (PPV) in leaves and flowers of plum, damson, myrobalan, blackthorn, apricot and peach trees was determined by enzyme-linked immunosorbent assay (ELISA) and expressed as the lowest dilution with positive reaction. Significant differences in relative PPV concentration were found in leaves among individual Prunus species naturally or artificially infected with the virus. The highest relative PPV concentration was found in blackthorns (7.81 x 10(-4)), common plum and apricot (1.56 x 10(-3) for the both latters). Wild growing PPV-infected plums and blackthorns can be considered equally important source of sharka infection as PPV-susceptible cultivars of plums, apricots and peaches. High PPV concentration in flowers is of diagnostical value. High variability of relative PPV concentration was observed inside the species among individual cultivars. Susceptible cultivars were characteristic by high relative PPV concentration, e.g. apricot cvs. Vegama (9.8 x 10(-5)) and Velkopavlovická (1.95 x 10(-4)), and peach cvs. Maria Emilia (7.81 x 10(-4)) and Harbinger (1.56 x 10(-3)). On the other hand, cultivars resistant to PPV were characteristic by very low relative PPV concentration, e.g. apricot cv. Stark Early Orange (5 x 10(-2)) and peach cvs. Envoy (5 x 10(-2)) and Favorita Morettini (2.5 x 10(-2)). The highest relative PPV concentration was found in young trees newly infected with PPV.
采用酶联免疫吸附测定（ELISA）法测定了李树、西洋李、樱桃李、黑刺李、杏树和桃树叶片及花朵中李痘病毒（PPV）的相对浓度，并将其表示为出现阳性反应的最低稀释度。在自然或人工感染该病毒的各个李属物种的叶片中，发现PPV相对浓度存在显著差异。黑刺李中的PPV相对浓度最高（7.81×10⁻⁴），普通李和杏树中的PPV相对浓度相同（均为1.56×10⁻³）。野生的感染PPV的李树和黑刺李可被视为与易感PPV的李、杏和桃品种同等重要的李痘病感染源。花朵中高浓度的PPV具有诊断价值。在各个品种的物种内部，观察到PPV相对浓度存在高度变异性。易感品种的特征是PPV相对浓度较高，例如杏品种Vegama（9.8×10⁻⁵）和Velkopavlovická（1.95×10⁻⁴），以及桃品种Maria Emilia（7.81×10⁻⁴）和Harbinger（1.56×10⁻³）。另一方面，抗PPV品种的特征是PPV相对浓度非常低，例如杏品种Stark Early Orange（5×10⁻²）以及桃品种Envoy（5×10⁻²）和Favorita Morettini（2.5×10⁻²）。新感染PPV的幼树中PPV相对浓度最高。
