Nemirov K, Vapalahti O, Lundkvist A, Vasilenko V, Golovljova I, Plyusnina A, Niemimaa J, Laakkonen J, Henttonen H, Vaheri A, Plyusnin A
J Gen Virol. 1999 Feb;80 ( Pt 2):371-379. doi: 10.1099/0022-1317-80-2-371.
Dobrava hantavirus (DOB) was isolated from the striped field mouse (Apodemus agrarius) trapped on Saaremaa Island, Estonia, and its genetic and antigenic characteristics were subsequently analysed. Phylogenetic analysis showed that the Estonian DOB strain, together with several wild strains carried by Apodemus agrarius, forms a well-supported lineage within the DOB clade. The topography of the trees calculated for the S, M and L nucleotide sequences of the Estonian DOB suggests a similar evolutionary history for all three genes of this virus and, therefore, the absence of heterologous reassortment in its evolution. A cross-neutralization comparison of the Estonian virus with the prototype DOB, isolated from a yellow-necked mouse (A. flavicollis) in Slovenia, revealed 2- to 4-fold differences in the end-point titres of rabbit and human antisera. When studied with a panel of 25 monoclonal antibodies (MAbs), the Estonian and Slovenian DOB isolates showed similar antigenic patterns that could be distinguished by two MAbs. Genetic comparison showed sequence differences in all three genome segments of the two DOB isolates, including an additional N-glycosylation site in the deduced sequence of the G2 protein from the Estonian virus. Whether any of these mutations relates to the different rodent hosts rather than to the distant geographical origin of the two isolates remains to be resolved. Taken together, our observations suggest that A. agrarius, which is known to harbour Hantaan virus in Asia, carries another hantavirus, DOB, in north-east Europe.
多布拉伐汉坦病毒（DOB）是从爱沙尼亚萨雷马岛捕获的条纹田鼠（黑线姬鼠）中分离出来的，随后对其遗传和抗原特性进行了分析。系统发育分析表明，爱沙尼亚DOB毒株与黑线姬鼠携带的几种野生毒株一起，在DOB进化枝中形成了一个得到充分支持的谱系。为爱沙尼亚DOB的S、M和L核苷酸序列计算的树形图表明，该病毒的所有三个基因都有相似的进化历史，因此，其进化过程中不存在异源重配。将爱沙尼亚病毒与从斯洛文尼亚黄颈鼠（黄颈姬鼠）中分离出的原型DOB进行交叉中和比较，发现兔和人抗血清的终点滴度存在2至4倍的差异。当用一组25种单克隆抗体（MAb）进行研究时，爱沙尼亚和斯洛文尼亚的DOB分离株显示出相似的抗原模式，可通过两种MAb进行区分。遗传比较显示，两种DOB分离株的所有三个基因组片段都存在序列差异，包括爱沙尼亚病毒G2蛋白推导序列中一个额外的N-糖基化位点。这些突变是否与不同的啮齿动物宿主有关，而不是与这两种分离株的遥远地理起源有关，仍有待解决。综上所述，我们的观察结果表明，在亚洲已知携带汉坦病毒的黑线姬鼠在欧洲东北部携带另一种汉坦病毒——DOB。
