Avsic-Zupanc T, Toney A, Anderson K, Chu Y K, Schmaljohn C
Virology Division, US Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
J Gen Virol. 1995 Nov;76 ( Pt 11):2801-8. doi: 10.1099/0022-1317-76-11-2801.
We examined the genetic and antigenic properties of Dobrava (DOB) virus, a hantavirus associated with severe haemorrhagic fever with renal syndrome in Europe. Cloning and sequence analyses revealed the DOB M segment to consist of 3644 nucleotides, with a coding capacity of 1134 amino acids in the virus complementary-sense RNA (cRNA). Seven potential asparagine-linked glycosylation sites were identified in the M segment gene product, one in the G2 and six in the G1 coding regions. The S segment is 1667 nucleotides long, and has a single ORF in the cRNA capable of encoding a protein of 428 amino acids. Phylogenetic comparisons of the M and S segments of DOB virus to those of other hantaviruses indicated that DOB virus is similar to, but clearly distinct from Hantaan (HTN) and Seoul (SEO) viruses. Certain G2-specific, but not G1-specific monoclonal antibodies to HTN virus reacted to the same titre with DOB and homologous viral antigen. Plaque-reduction neutralization tests indicated that, of the sera tested, only antisera to SEO virus were able to neutralize DOB virus to a titre greater than 1:10; however, this neutralization titre was eightfold lower than that observed with homologous SEO virus. The data reported here confirm that DOB virus is a unique species in the Hantavirus genus, family Bunyaviridae.
我们研究了多布拉瓦（DOB）病毒的遗传和抗原特性，该病毒是一种在欧洲与严重肾综合征出血热相关的汉坦病毒。克隆和序列分析显示，DOB病毒的M片段由3644个核苷酸组成，在病毒互补义RNA（cRNA）中编码能力为1134个氨基酸。在M片段基因产物中鉴定出7个潜在的天冬酰胺连接的糖基化位点，1个在G2中，6个在G1编码区域。S片段长1667个核苷酸，在cRNA中有一个单一的开放阅读框，能够编码一个428个氨基酸的蛋白质。将DOB病毒的M和S片段与其他汉坦病毒的相应片段进行系统发育比较表明，DOB病毒与汉滩（HTN）病毒和汉城（SEO）病毒相似，但明显不同。某些针对HTN病毒的G2特异性而非G1特异性单克隆抗体与DOB病毒和同源病毒抗原的反应滴度相同。空斑减少中和试验表明，在所测试的血清中，只有针对SEO病毒的抗血清能够将DOB病毒中和至大于1:10的滴度；然而，该中和滴度比同源SEO病毒观察到的滴度低8倍。此处报告的数据证实，DOB病毒是布尼亚病毒科汉坦病毒属中的一个独特物种。
