Mehta A D, Rief M, Spudich J A, Smith D A, Simmons R M
Department of Biochemistry B400, Stanford University School of Medicine, Stanford, CA 94305-5307, USA.
Science. 1999 Mar 12;283(5408):1689-95. doi: 10.1126/science.283.5408.1689.
Single-molecule observation and manipulation have come of age. With the advent of optical tweezers and other methods for probing and imaging single molecules, investigators have circumvented the model-dependent extrapolation from ensemble assays that has been the hallmark of classical biochemistry and biophysics. In recent years, there have been important advances in the understanding of how motor proteins work. The range of these technologies has also started to expand into areas such as DNA transcription and protein folding. Here, recent experiments with rotary motors, linear motors, RNA polymerase, and titin are described.
单分子观察与操控已走向成熟。随着光镊及其他用于探测和成像单分子的方法的出现，研究人员已规避了传统生物化学和生物物理学标志性的、依赖模型的从整体测定进行的外推法。近年来，在理解驱动蛋白如何工作方面取得了重要进展。这些技术的应用范围也已开始扩展到DNA转录和蛋白质折叠等领域。在此，将介绍近期关于旋转马达、线性马达、RNA聚合酶和肌联蛋白的实验。
