Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil.
J Bacteriol. 2011 Jan;193(1):323-4. doi: 10.1128/JB.01211-10. Epub 2010 Oct 29.
This work reports the completion and annotation of the genome sequence of Corynebacterium pseudotuberculosis I19, isolated from an Israeli dairy cow with severe clinical mastitis. To present the whole-genome sequence, a de novo assembly approach using 33 million short (25-bp) mate-paired SOLiD reads only was applied. Furthermore, the automatic, functional, and manual annotations were attained with the use of several algorithms in a multistep process.
本工作报道了从以色列患有严重临床乳腺炎的奶牛中分离到的化脓隐秘杆菌 I19 基因组序列的完成和注释。为了呈现全基因组序列,仅使用了 3300 万个短(25bp)配对 SOLiD 读取进行从头组装方法。此外,还使用了几种算法在多步过程中实现了自动、功能和手动注释。