在意大利分离出的耐多药结核分枝杆菌中的rpoB基因突变
rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy.
作者信息
Pozzi G, Meloni M, Iona E, Orrù G, Thoresen O F, Ricci M L, Oggioni M R, Fattorini L, Orefici G
机构信息
Sezione di Microbiologia, Dipartimento di Biologia Molecolare, Università di Siena, Siena, Italy.
出版信息
J Clin Microbiol. 1999 Apr;37(4):1197-9. doi: 10.1128/JCM.37.4.1197-1199.1999.
Abstract
Mutations of rpoB associated with rifampin resistance were studied in 37 multidrug-resistant (MDR) clinical strains of Mycobacterium tuberculosis isolated in Italy. At least one mutated codon was found in each MDR strain. It was always a single-base substitution leading to an amino acid change. Nine different rpoB alleles, three of which had not been reported before, were found. The relative frequencies of specific mutations in this sample were different from those previously reported from different geographical areas, since 22 strains (59.5%) carried the mutated codon TTG in position 531 (Ser-->Leu) and 11 (29.7%) had GAC in position 526 (His-->Asp).
摘要
对从意大利分离出的37株耐多药结核分枝杆菌临床菌株中与利福平耐药相关的rpoB突变进行了研究。在每株耐多药菌株中均发现至少一个突变密码子。其总是导致氨基酸改变的单碱基替换。发现了9种不同的rpoB等位基因，其中3种此前未曾报道。该样本中特定突变的相对频率与之前不同地理区域报道的频率不同，因为22株菌株（59.5%）在第531位携带突变密码子TTG（丝氨酸→亮氨酸），11株（29.7%）在第526位有GAC（组氨酸→天冬氨酸）。
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