免疫扩散法与酶联免疫吸附测定法检测四种曲霉菌属抗体的比较
Comparison of immunodiffusion and enzyme linked immunosorbent assay for antibodies to four Aspergillus species.
作者信息
Froudist J H, Harnett G B, McAleer R
机构信息
Medical Mycology Laboratory, State Health Laboratories, Perth, Western Australia.
出版信息
J Clin Pathol. 1989 Nov;42(11):1215-21. doi: 10.1136/jcp.42.11.1215.
Antigenic extracts were prepared from Aspergillus fumigatus, A niger, A flavus and A terreus for use in enzyme linked immunosorbent assay (ELISA) and immunodiffusion (ID) tests for Aspergillus antibodies to determine whether the use of antigenic extracts from species other than A fumigatus increased the sensitivity of the ELISA. ELISA titres correlated well with positive ID tests. Patient titres by ELISA were significantly higher than control titres for all species. Patient titres to A niger were also significantly higher than titres to the other species. Total number of ID bands to A fumigatus correlated significantly with anti-A fumigatus ELISA titres. It is concluded that the use of antigenic extracts from species other than A fumigatus improves the sensitivity of the ELISA.
从烟曲霉、黑曲霉、黄曲霉和土曲霉中制备抗原提取物，用于酶联免疫吸附测定（ELISA）和免疫扩散（ID）试验，以检测曲霉抗体，从而确定使用烟曲霉以外其他菌种的抗原提取物是否能提高ELISA的灵敏度。ELISA滴度与阳性ID试验结果相关性良好。所有菌种的患者ELISA滴度均显著高于对照滴度。患者对黑曲霉的滴度也显著高于对其他菌种的滴度。针对烟曲霉的ID条带总数与抗烟曲霉ELISA滴度显著相关。得出的结论是，使用烟曲霉以外其他菌种的抗原提取物可提高ELISA的灵敏度。
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