González L, Buxton D, Atxaerandio R, Aduriz G, Maley S, Marco J C, Cuervo L A
Departamento de Patología Animal, SIMA-CIMA, Gobierno Vasco, Derio, Spain.
Vet Rec. 1999 Feb 6;144(6):145-50. doi: 10.1136/vr.144.6.145.
During 1996, aborted bovine fetuses from dairy herds in norther Spain were examined by histopathological, immunohistochemical and serological methods for evidence of infection by Neospora caninum. Microscopical brain changes consistent with those of neosporosis were detected in 36 of 81 fetuses and specific antibodies (IFAT titres > or = 1/16) in 32 of 63. Eight fetuses with neurological lesions were seronegative, and eight without lesions were seropositive. In 25 of 34 fetal brains with lesions consistent with published descriptions of neosporosis, immunohistochemical analysis revealed the presence of N caninum antigen. Depending on the diagnostic criteria, the prevalence of neosporosis related to bovine abortion was between 32 and 57 per cent of the fetuses submitted and between 33 and 58 per cent of the herds involved. Data on the geographical, seasonal and fetal age distribution of the infection are given for the fetuses examined and the value of the different diagnostic methods is discussed.
1996年期间，采用组织病理学、免疫组织化学和血清学方法对西班牙北部奶牛场的流产牛胎儿进行检查，以寻找犬新孢子虫感染的证据。在81个胎儿中的36个检测到与新孢子虫病相符的微观脑部变化，在63个中的32个检测到特异性抗体（间接荧光抗体试验滴度≥1/16）。8个有神经病变的胎儿血清学呈阴性，8个无病变的胎儿血清学呈阳性。在34个有与已发表的新孢子虫病描述相符病变的胎儿大脑中，25个经免疫组织化学分析显示存在犬新孢子虫抗原。根据诊断标准，与牛流产相关的新孢子虫病患病率在所提交胎儿中为32%至57%，在所涉及牛群中为33%至58%。给出了所检查胎儿感染的地理、季节和胎龄分布数据，并讨论了不同诊断方法的价值。
