碱性成纤维细胞生长因子在小鼠进行胶质细胞特异性基因转移后可诱导细胞迁移和增殖。
Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice.
作者信息
Holland E C, Varmus H E
机构信息
Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1218-23. doi: 10.1073/pnas.95.3.1218.
Abstract
Basic fibroblast growth factor (bFGF) is overexpressed in most high-grade human gliomas, implying that it is involved in the pathogenesis of these tumors. To assess the biological effect of inappropriate production of bFGF in normal astrocytes, we developed a system for glia-specific gene transfer in transgenic mice. A transgene encoding the receptor for subgroup A avian leukosis virus and controlled by the astrocyte-specific glial fibrillary acidic protein promoter permits efficient glia-specific transfer of genes carried by subgroup A avian leukosis virus vectors. With this system, we have demonstrated that bFGF induces proliferation and migration of glial cells in vivo, without the induction of tumors.
摘要
碱性成纤维细胞生长因子（bFGF）在大多数高级别人类胶质瘤中过度表达，这表明它参与了这些肿瘤的发病机制。为了评估正常星形胶质细胞中bFGF异常产生的生物学效应，我们在转基因小鼠中开发了一种用于胶质细胞特异性基因转移的系统。一个由星形胶质细胞特异性胶质纤维酸性蛋白启动子控制的、编码A亚群禽白血病病毒受体的转基因，允许A亚群禽白血病病毒载体携带的基因进行高效的胶质细胞特异性转移。利用这个系统，我们已经证明bFGF在体内可诱导胶质细胞增殖和迁移，而不会诱导肿瘤形成。
相似文献
1
Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice.碱性成纤维细胞生长因子在小鼠进行胶质细胞特异性基因转移后可诱导细胞迁移和增殖。
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1218-23. doi: 10.1073/pnas.95.3.1218.
2
The potential role of basic fibroblast growth factor in the transformation of cultured primary human fetal astrocytes and the proliferation of human glioma (U-87) cells.碱性成纤维细胞生长因子在人胎儿原代星形胶质细胞培养转化及人神经胶质瘤（U-87）细胞增殖中的潜在作用。
Neurosurgery. 1995 Oct;37(4):723-30; discussion 730-2. doi: 10.1227/00006123-199510000-00017.
3
PDGF autocrine stimulation dedifferentiates cultured astrocytes and induces oligodendrogliomas and oligoastrocytomas from neural progenitors and astrocytes in vivo.血小板衍生生长因子自分泌刺激可使培养的星形胶质细胞去分化，并在体内诱导神经祖细胞和星形胶质细胞产生少突胶质细胞瘤和少突星形细胞瘤。
Genes Dev. 2001 Aug 1;15(15):1913-25. doi: 10.1101/gad.903001.
4
Neuro-glia interaction effects on GFAP gene: a novel role for transforming growth factor-beta1.神经胶质细胞相互作用对胶质纤维酸性蛋白基因的影响：转化生长因子-β1的新作用
Eur J Neurosci. 2002 Dec;16(11):2059-69. doi: 10.1046/j.1460-9568.2002.02283.x.
5
Promoter regions involved in density-dependent regulation of basic fibroblast growth factor gene expression in human astrocytic cells.参与人星形胶质细胞中碱性成纤维细胞生长因子基因表达密度依赖性调控的启动子区域。
Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2470-5. doi: 10.1073/pnas.93.6.2470.
6
A constitutively active epidermal growth factor receptor cooperates with disruption of G1 cell-cycle arrest pathways to induce glioma-like lesions in mice.一种组成型激活的表皮生长因子受体与G1期细胞周期阻滞途径的破坏协同作用，在小鼠中诱导胶质瘤样病变。
Genes Dev. 1998 Dec 1;12(23):3675-85. doi: 10.1101/gad.12.23.3675.
7
Tumor necrosis factor-alpha and basic fibroblast growth factor decrease glial fibrillary acidic protein and its encoding mRNA in astrocyte cultures and glioblastoma cells.肿瘤坏死因子-α和碱性成纤维细胞生长因子可降低星形胶质细胞培养物和胶质母细胞瘤细胞中胶质纤维酸性蛋白及其编码信使核糖核酸的水平。
J Neurochem. 1995 Dec;65(6):2716-24. doi: 10.1046/j.1471-4159.1995.65062716.x.
8
Basic fibroblast growth factor (bFGF) acts on both neurons and glia to mediate the neurotrophic effects of astrocytes on LHRH neurons in culture.碱性成纤维细胞生长因子（bFGF）作用于神经元和神经胶质细胞，以介导星形胶质细胞对培养中的促性腺激素释放激素（LHRH）神经元的神经营养作用。
Synapse. 2000 Jun 15;36(4):233-53. doi: 10.1002/(SICI)1098-2396(20000615)36:4<233::AID-SYN1>3.0.CO;2-I.
9
Development of a flexible and specific gene delivery system for production of murine tumor models.用于构建小鼠肿瘤模型的灵活且特异性基因递送系统的开发。
Oncogene. 1999 Sep 20;18(38):5253-60. doi: 10.1038/sj.onc.1203087.
10
Glial fibrillary acidic protein gene promoter is differently modulated by transforming growth factor-beta 1 in astrocytes from distinct brain regions.胶质纤维酸性蛋白基因启动子在来自不同脑区的星形胶质细胞中受转化生长因子-β1的调控方式不同。
Eur J Neurosci. 2004 Apr;19(7):1721-30. doi: 10.1111/j.1460-9568.2004.03249.x.
引用本文的文献
1
Single-cell lineage tracing techniques in hematology: unraveling the cellular narrative.血液学中的单细胞谱系追踪技术：揭示细胞的故事
J Transl Med. 2025 Mar 4;23(1):270. doi: 10.1186/s12967-025-06318-4.
2
The lives of cells, recorded.细胞的生命，被记录下来。
Nat Rev Genet. 2025 Mar;26(3):203-222. doi: 10.1038/s41576-024-00788-w. Epub 2024 Nov 25.
3
Rodent models of tumours of the central nervous system.中枢神经系统肿瘤的啮齿动物模型
Mol Oncol. 2024 Dec;18(12):2842-2870. doi: 10.1002/1878-0261.13729. Epub 2024 Sep 26.
4
In Vitro Assessment of Wound-Healing Efficacy of Stabilized Basic Fibroblast Growth Factor (FGF-2) Solutions.稳定型碱性成纤维细胞生长因子（FGF-2）溶液伤口愈合功效的体外评估
Pharmaceuticals (Basel). 2024 Feb 14;17(2):247. doi: 10.3390/ph17020247.
5
FABP5 Inhibition against -Mutant Therapy Resistant Prostate Cancer.脂肪酸结合蛋白5（FABP5）对突变型抗治疗性前列腺癌的抑制作用
Cancers (Basel). 2023 Dec 21;16(1):60. doi: 10.3390/cancers16010060.
6
Secured delivery of basic fibroblast growth factor using human serum albumin-based protein nanoparticles for enhanced wound healing and regeneration.利用人血清白蛋白为基础的蛋白纳米粒递运碱性成纤维细胞生长因子促进伤口愈合和再生。
J Nanobiotechnology. 2023 Sep 2;21(1):310. doi: 10.1186/s12951-023-02053-4.
7
Remote neuronal activity drives glioma progression through SEMA4F.远程神经元活动通过 SEMA4F 驱动神经胶质瘤进展。
Nature. 2023 Jul;619(7971):844-850. doi: 10.1038/s41586-023-06267-2. Epub 2023 Jun 28.
8
Efficient cancer modeling through CRISPR-Cas9/HDR-based somatic precision gene editing in mice.通过 CRISPR-Cas9/HDR 为基础的体细胞精确基因编辑在小鼠中实现高效癌症建模。
Sci Adv. 2023 May 12;9(19):eade0059. doi: 10.1126/sciadv.ade0059.
9
Remote neuronal activity drives glioma infiltration via Sema4f.远程神经元活动通过Sema4f驱动胶质瘤浸润。
bioRxiv. 2023 Mar 16:2023.03.15.532832. doi: 10.1101/2023.03.15.532832.
10
Novel genetically engineered H3.3G34R model reveals cooperation with ATRX loss in upregulation of cluster genes and promotion of neuronal lineage.新型基因工程H3.3G34R模型揭示了与ATRX缺失在簇基因上调和神经元谱系促进中的协同作用。
Neurooncol Adv. 2023 Jan 6;5(1):vdad003. doi: 10.1093/noajnl/vdad003. eCollection 2023 Jan-Dec.
本文引用的文献
1
Genetic mapping of the cloned subgroup A avian sarcoma and leukosis virus receptor gene to the TVA locus.克隆的A亚群禽肉瘤和白血病病毒受体基因的遗传定位至TVA基因座。
J Virol. 1998 Mar;72(3):2505-8. doi: 10.1128/JVI.72.3.2505-2508.1998.
2
Transplanted oligodendrocyte progenitor cells expressing a dominant-negative FGF receptor transgene fail to migrate in vivo.表达显性负性成纤维细胞生长因子受体转基因的移植少突胶质前体细胞在体内无法迁移。
J Neurosci. 1997 Dec 1;17(23):9122-32. doi: 10.1523/JNEUROSCI.17-23-09122.1997.
3
Development and malignant progression of astrocytomas in GFAP-v-src transgenic mice.GFAP-v-src转基因小鼠星形细胞瘤的发生与恶性进展
Oncogene. 1997 May 1;14(17):2005-13. doi: 10.1038/sj.onc.1201168.
4
PTEN, a putative protein tyrosine phosphatase gene mutated in human brain, breast, and prostate cancer.PTEN是一种假定的蛋白酪氨酸磷酸酶基因，在人类脑癌、乳腺癌和前列腺癌中发生突变。
Science. 1997 Mar 28;275(5308):1943-7. doi: 10.1126/science.275.5308.1943.
5
Molecular genetics and molecular biology advances in brain tumors.脑肿瘤的分子遗传学与分子生物学进展
Curr Opin Oncol. 1996 May;8(3):188-95. doi: 10.1097/00001622-199605000-00004.
6
The potential role of basic fibroblast growth factor in the transformation of cultured primary human fetal astrocytes and the proliferation of human glioma (U-87) cells.碱性成纤维细胞生长因子在人胎儿原代星形胶质细胞培养转化及人神经胶质瘤（U-87）细胞增殖中的潜在作用。
Neurosurgery. 1995 Oct;37(4):723-30; discussion 730-2. doi: 10.1227/00006123-199510000-00017.
7
A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor.A亚群劳氏肉瘤病毒的一种受体与低密度脂蛋白受体相关。
Cell. 1993 Sep 24;74(6):1043-51. doi: 10.1016/0092-8674(93)90726-7.
8
Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses.一种使鸡对A亚群禽白血病和肉瘤病毒感染易感的基因的分离。
J Virol. 1993 Apr;67(4):1811-6. doi: 10.1128/JVI.67.4.1811-1816.1993.
9
GFAP promoter directs astrocyte-specific expression in transgenic mice.胶质纤维酸性蛋白（GFAP）启动子在转基因小鼠中指导星形胶质细胞特异性表达。
J Neurosci. 1994 Mar;14(3 Pt 1):1030-7. doi: 10.1523/JNEUROSCI.14-03-01030.1994.
Zotero 插件