Holland E C, Varmus H E
Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1218-23. doi: 10.1073/pnas.95.3.1218.
Basic fibroblast growth factor (bFGF) is overexpressed in most high-grade human gliomas, implying that it is involved in the pathogenesis of these tumors. To assess the biological effect of inappropriate production of bFGF in normal astrocytes, we developed a system for glia-specific gene transfer in transgenic mice. A transgene encoding the receptor for subgroup A avian leukosis virus and controlled by the astrocyte-specific glial fibrillary acidic protein promoter permits efficient glia-specific transfer of genes carried by subgroup A avian leukosis virus vectors. With this system, we have demonstrated that bFGF induces proliferation and migration of glial cells in vivo, without the induction of tumors.
碱性成纤维细胞生长因子（bFGF）在大多数高级别人类胶质瘤中过度表达，这表明它参与了这些肿瘤的发病机制。为了评估正常星形胶质细胞中bFGF异常产生的生物学效应，我们在转基因小鼠中开发了一种用于胶质细胞特异性基因转移的系统。一个由星形胶质细胞特异性胶质纤维酸性蛋白启动子控制的、编码A亚群禽白血病病毒受体的转基因，允许A亚群禽白血病病毒载体携带的基因进行高效的胶质细胞特异性转移。利用这个系统，我们已经证明bFGF在体内可诱导胶质细胞增殖和迁移，而不会诱导肿瘤形成。
