产吲哚金黄杆菌（黄杆菌属）中一种碳青霉烯水解β-内酰胺酶的分子特征
Molecular characterization of a carbapenem-hydrolyzing beta-lactamase from Chryseobacterium (Flavobacterium) indologenes.
作者信息
Bellais S, Léotard S, Poirel L, Naas T, Nordmann P
机构信息
Service de Bactériologie-Virologie, Hôpital de Bicêtre, Faculté de Médecine Paris-Sud, Le Kremlin-Bicêtre, France.
出版信息
FEMS Microbiol Lett. 1999 Feb 15;171(2):127-32. doi: 10.1111/j.1574-6968.1999.tb13422.x.
Chryseobacterium (Flavobacterium) indologenes 001 clinical strain was resistant to several beta-lactam classes including carbapenems. Shotgun cloning experiments of Sau3AI restricted genomic DNA of C. indologenes 001 into pBKCMV cloning vector followed by transformation into Escherichia coli DH10B gave one recombinant plasmid possessing a 4.2-kb DNA insert. It encoded a pI 7.2 beta-lactamase of 239 amino acids (IND-1) which is a metallo-enzyme with a broad spectrum beta-lactam hydrolysis profile. This class B carbapenem-hydrolyzing beta-lactamase shares the highest identity (43%) with BlaB from C. meningosepticum, thus showing heterogeneity of carbapenem-hydrolyzing beta-lactamases in Chryseobacterium spp.
产吲哚金黄杆菌（黄杆菌属）001临床菌株对包括碳青霉烯类在内的多种β-内酰胺类抗生素耐药。用Sau3AI对产吲哚金黄杆菌001的基因组DNA进行酶切，将酶切后的片段克隆到pBKCMV克隆载体中，然后转化到大肠杆菌DH10B中，通过鸟枪法克隆实验得到了一个含有4.2 kb DNA插入片段的重组质粒。该质粒编码一种由239个氨基酸组成的pI 7.2的β-内酰胺酶（IND-1），它是一种具有广谱β-内酰胺水解谱的金属酶。这种B类碳青霉烯水解β-内酰胺酶与脑膜败血金黄杆菌的BlaB具有最高的同源性（43%），从而表明产吲哚金黄杆菌属中碳青霉烯水解β-内酰胺酶具有异质性。
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