Burbaeva G Sh, Savushkina O K, Dmitriev A D
Vestn Ross Akad Med Nauk. 1999(1):20-4.
The paper analyzes the authors' own findings and the data available in the literature on the intensity, site, and possible causes of impairment of the creatine-creatine phosphate system of brain energy metabolism in mental diseases, such as Alzheimer's disease (AD) and schizophrenia. Examining the level of cytosolic BB creatine kinase in postmortem AD and schizophrenic's brain structures showed a significant decrease in BB creatine kinase as compared with the similar control brain structures. There was the maximum decline in AD cases. It was considerable as compared with both the control and schizophrenic groups (p < 0.01). The decrement was revealed by various techniques, including the determination of activity, immunological responsiveness and the analysis of two-dimensional protein maps. Immunocytochemical investigation indicated a decrease in responses to BB creatine kinase, mainly in astrocytes. The reduction in cytosolic BB creatine kinase levels is not a result of age, postmortem delay, or psychotic therapy. The causes of lower BB creatine kinase levels in the cell cytosol of the postmortem brain in mental pathology are discussed. The decrement in cytosolic BB creatine kinase in AD and schizophrenia occurs not only in the brain, but also in the peripheral tissues which contain BB creatine kinase. In all cases, it is greater in AD than in schizophrenia. Using immunosorbents with monoclonal antibodies to M-creatine kinase and to B-creatine kinase subunits makes it possible detect BB-creatine kinase in the extracts of human peripheral lymphocytes and platelets. A study of whether there is a relationship between the clinical data of mental patients and the level of BB creatine kinase in their blood elements is assumed to be useful in evaluating BB creatine kinase as a prognostic/diagnostic marker of mental diseases.
本文分析了作者自身的研究结果以及文献中关于精神疾病（如阿尔茨海默病（AD）和精神分裂症）中脑能量代谢的肌酸-磷酸肌酸系统损伤的强度、部位及可能原因的数据。对AD患者和精神分裂症患者死后大脑结构中胞质BB型肌酸激酶水平的检测显示，与相似的对照大脑结构相比，BB型肌酸激酶显著降低。AD病例中的下降最为明显。与对照组和精神分裂症组相比，下降幅度都很大（p<0.01）。通过多种技术揭示了这种下降，包括活性测定、免疫反应性测定以及二维蛋白质图谱分析。免疫细胞化学研究表明，对BB型肌酸激酶的反应降低，主要发生在星形胶质细胞中。胞质BB型肌酸激酶水平的降低不是年龄、死后延迟或精神治疗的结果。讨论了精神病理学中死后大脑细胞质中BB型肌酸激酶水平降低的原因。AD和精神分裂症中胞质BB型肌酸激酶的降低不仅发生在大脑中，也发生在含有BB型肌酸激酶的外周组织中。在所有情况下，AD中的降低幅度都大于精神分裂症。使用针对M型肌酸激酶和B型肌酸激酶亚基的单克隆抗体免疫吸附剂，可以在人外周淋巴细胞和血小板提取物中检测到BB型肌酸激酶。研究精神疾病患者的临床数据与其血液成分中BB型肌酸激酶水平之间是否存在关联，被认为有助于将BB型肌酸激酶评估为精神疾病的预后/诊断标志物。
