Aksenova M V, Karaseva N S, Makarenko I G, Burbaeva G Sh
Biull Eksp Biol Med. 1993 Feb;115(2):153-5.
This work is devoted to the study of CPK BB content in nuclear fraction of nervous cells in normal brain and in brains of mental (schizophrenia and Alzheimer disease) patients. With the help of the immunocytochemistry and immunoblotting was detected, that the nuclear membrane fraction of brain contains significant amount of CPK BB. On the contrary, in the nuclear membrane fractions of schizophrenic and Alzheimer disease brains the content of this isoenzyme decreased. Therefore in the brain of schizophrenic and Alzheimer disease patients the content of CPK BB decreased not only in cytosolic fractions, but also in the fractions of nuclear membranes. We demonstrated also, that cytosolic CPK BB associates not only with nuclear membranes, but with synaptosomal and microsomal fractions and mitochondrias of normal brain cells.
这项工作致力于研究正常大脑以及患有精神疾病（精神分裂症和阿尔茨海默病）患者大脑中神经细胞核部分的肌酸磷酸激酶BB（CPK BB）含量。借助免疫细胞化学和免疫印迹法检测到，大脑的核膜部分含有大量的CPK BB。相反，在精神分裂症患者和阿尔茨海默病患者大脑的核膜部分，这种同工酶的含量降低。因此，在精神分裂症患者和阿尔茨海默病患者的大脑中，CPK BB的含量不仅在胞质部分降低，而且在核膜部分也降低。我们还证明，胞质CPK BB不仅与核膜相关，而且与正常脑细胞的突触体、微粒体部分以及线粒体相关。
