具有C端硫代羧酸盐的重组蛋白的过表达：对蛋白质半合成和硫胺素生物合成的影响。
Overexpression of recombinant proteins with a C-terminal thiocarboxylate: implications for protein semisynthesis and thiamin biosynthesis.
作者信息
Kinsland C, Taylor S V, Kelleher N L, McLafferty F W, Begley T P
机构信息
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
出版信息
Protein Sci. 1998 Aug;7(8):1839-42. doi: 10.1002/pro.5560070821.
Abstract
A facile and rapid method for the production of protein C-terminal thiocarboxylates on DNA-encoded polypeptides is described. This method, which relies on the mechanism of the cleavage reaction of intein-containing fusion proteins, can produce multi-milligram quantities of protein C-terminal thiocarboxylate quickly and inexpensively. The utility of this method for protein semisynthesis and implications for studies on the biosynthesis of thiamin are discussed.
摘要
描述了一种在DNA编码多肽上制备蛋白质C端硫代羧酸盐的简便快速方法。该方法基于含内含肽融合蛋白的切割反应机制，能够快速且低成本地制备多毫克量的蛋白质C端硫代羧酸盐。讨论了该方法在蛋白质半合成中的应用以及对硫胺素生物合成研究的意义。
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