棘孢曲霉β-1,4-半乳聚糖酶的结晶及初步X射线研究
Crystallization and preliminary X-ray studies of beta-1, 4-galactanase from Aspergillus aculeatus.
作者信息
Ryttersgaard C, Poulsen J, Christgau S, Sandal T, Dalbøge H, Larsen S
机构信息
Centre for Crystallographic Studies, H. C. Orsted Institute, University of Copenhagen, Universitetsparken 5, Copenhagen DK-2100 Denmark.
出版信息
Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):929-30. doi: 10.1107/s0907444998001073.
Recombinant beta-1,4-galactanase from Aspergillus aculeatus has been crystallized and characterized by X-ray diffraction. Crystals were obtained in hanging drops by vapour-diffusion under the conditions 30% PEG 400, 0.2 M CaCl2 and 0.1 M Na HEPES buffered to pH 7.5. The crystals diffract to 2.3 A resolution and belong to one of the orthorhombic space groups I222 or I212121. The unit-cell dimensions are a = 60.42, b = 88.94 and c = 129.08 A. With one molecule in the asymmetric unit, the corresponding solvent content is approximately 48%.
来自棘孢曲霉的重组β-1,4-半乳聚糖酶已通过X射线衍射进行了结晶和表征。在30%聚乙二醇400、0.2 M氯化钙和用0.1 M Na HEPES缓冲至pH 7.5的条件下，通过气相扩散在悬滴中获得晶体。这些晶体的衍射分辨率为2.3 Å，属于正交空间群I222或I212121之一。晶胞参数为a = 60.42、b = 88.94和c = 129.08 Å。不对称单元中有一个分子，相应的溶剂含量约为48%。
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