Komaki H, Nemoto A, Tanaka Y, Takagi H, Yazawa K, Mikami Y, Shigemori H, Kobayashi J, Ando A, Nagata Y
Research Laboratory, Higeta Shoyu Co., Ltd., Choshi, Chiba, Japan.
J Antibiot (Tokyo). 1999 Jan;52(1):13-9. doi: 10.7164/antibiotics.52.13.
A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC50 value was 0.057 microg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.
一种新型三环二萜类抗生素——巴西卡迪菌素A，是从巴西诺卡氏菌IFM 0406的培养液中分离得到的。该抗生素在小鼠混合淋巴细胞反应（MLR）检测系统中表现出免疫抑制活性，其IC50值为0.057微克/毫升。尽管在MLR检测系统中证实了环孢素A（CyA）对白细胞介素-2产生的抑制活性，但巴西卡迪菌素A没有这种活性。将巴西卡迪菌素A对各种人类细胞系的体外毒性测试结果与环孢素A的测试结果进行了比较。
